Abstract
The immunohistochemical features of 24 retinoblastoma specimens from 22 patients, 15 with unilateral and 7 with bilateral disease, were examined by the labelled streptavidin biotin (LSAB) method and compared with those of specimens from the remaining morphologically normal retina. In the normal retina, S-100 protein, glial fibrillary acidic protein (GFAP) and vimentin were detected in astrocytes and/or Müller cells. Neurofilament protein was seen in axons of the ganglion cells, synaptophysin was present in both plexiform layers, bcl-2 oncoprotein was seen in ganglion cells and bipolar cells, and neuron-specific enolase (NSE) was detected in ganglion cells, bipolar cells and photoreceptor cells and in their cell processes. While retinoblastoma (Rb) protein expression was noted in ganglion cells, bipolar cells, and some photoreceptor cells, p53 protein was not expressed at all. In all retinoblastomas, strong NSE expression and weak bcl-2 expression was observed in almost all tumour cells and synaptophysin was localized in rosette-forming cells, while tumour cells were devoid of S-100, GFAP, vimentin and neurofilament protein. These findings support the view that retinoblastomas are composed of neuron-committed cells. In addition, no Rb protein expression was detected in retinoblastomas, whereas p53 expression was found in 18 cases (75%).
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Benedict WF, Xu H-J, Hu S-X, Takahashi R (1990) Role of the retinoblastoma gene in the initiation and progression of human cancer. J Clin Invest 85:988–993
Cordon-Cardo C, Richon VM (1994) Expression of the retinoblastoma protein is regulated in normal tissues. Am J Pathol 144:500–510
Ewen ME, Sluss HK, Sherr CJ, Matsushime H, Kato J, Livingston DM (1993) Functional interactions of the retinoblastoma protein with mammalian D-type cyclins. Cell 73:487–497
Finlay CA (1992) p53 loss of function: implications for the processes of immortalization and tumorigenesis. BioEssays 14:557–559
Gallie BL, Squire JA, Goddard A, Dunn JM, Canton M, Hinton D, Zhu XP, Phillips RA (1990) Mechanism of oncogenesis in retinoblastoma. Lab Invest 62:394–408
He W, Hashimoto H, Tsuneyoshi M, Enjoji M, Inomata H (1992) A reassessment of histologic classification and an immunohistochemical study of 88 retinoblastomas. A special reference to the advent of bipolar-like cells. Cancer 70:2901–2908
Jiang Q, Lim R, Blodi FC (1984) Dual properties of cultured retinoblastoma cells: immunohistochemical characterization of neuronal and glial markers. Exp Eye Res 39:207–215
Katsetos CD, Herman MM, Frankfurter A, Uffer S, Perentes E, Rubinstein LJ (1991) Neuron-associated class III β-tubulin isotype, microtubule-associated protein 2, and synaptophysin in human retinoblastomas in situ. Further immunohistochemical observations on the Flexner-Wintersteiner rosettes. Lab Invest 64:45–54
Kivelä T (1986) Neuron-specific enolase in retinoblastoma. An immunohistochemical study. Acta Ophthalmol 64:19–25
Kivelä T, Tarkkanen A (1986) S-100 protein in retinoblastoma revisited. An immunohistochemical study. Acta Ophthalmol 64:664–673
Kivelä T, Tarkkanen A, Virtanen I (1986) Intermediate filaments in the human retina and retinoblastoma. An immunohistochemical study of vimentin, glial fibrillary acidic protein, and neurofilaments. Invest Ophthalmol Vis Sci 27:1075–1084
Kivelä T, Tarkkanen A, Virtanen I (1989) Synaptophysin in the human retina and retinoblastoma. An immunohistochemical and Western blotting study. Invest Ophthalmol Vis Sci 30:212–219
Kyritsis AP, Tsokos M, Triche TJ, Chader GJ (1986) Retinoblastoma: a primitive tumor with multipotential characteristics. Invest Ophthalmol Vis Sci 27:1760–1764
Lane JC, Klintworth GK (1983) A study of astrocytes in retinoblastomas using the immunoperoxidase technique and antibodies to glial fibrillary acidic protein. Am J Ophthalmol 95:197–207
LeBrun DP, Warnke RA, Cleary ML (1993) Expression of bcl-2 in fetal tissues suggests a role in morphogenesis. Am J Pathol 142:743–753
Perntes E, Herbort CP, Rubinstein LJ, Herman MM, Uffer S, Donoso LA, Collins VP (1987) Immunohistochemical characterization of human retinoblastomas in situ with multiple markers. Am J Ophthalmol 103:647–658
Popoff N, Ellsworth RM (1969) The fine structure of nuclear alterations in retinoblastoma and in the developing human retina: in vivo and in vitro observations. J Ultrastruct Res 29:535–549
Porter PL, Gown AM, Kramp SG, Coltrera ND (1992) Widespread p53 overexpression in human malignant tumors. An immunohistochemical study using methacarn-fixed, embedded tissue. Am J Pathol 140:145–153
Ramani P, Lu Q-L (1994) Expression of bcl-2 gene product in neuroblastoma. J Pathol (Lond) 172:273–278
Rodrigues MM, Wilson ME, Wiggert B, Krishna G, Chader GJ (1986) Retinoblastoma. A clinical, immunohistochemical, and electron microscopic case report. Ophthalmology 93:1010–1015
Rodrigues MM, Wiggert B, Shields J, Donoso L, Bardenstein D, Katz N, Friendly D, Chader G (1987) Retinoblastoma. Immunohistochemistry and cell differentiation. Ophthalmology 94:378–387
Tajima Y, Munakata S, Ishida Y, Nakajima T, Sugano I, Nagao K, Minoda K, Kondo Y (1994) Photoreceptor differentiation of retinoblastoma: an electron microscopic study of 29 retinoblastomas. Pathol Int 44:837–843
Tarlton JF, Easty DL (1990) Immunohistological characterisation of retinoblastoma and related ocular tissue. Br J Ophthalmol 74:144–149
Tso MO (1980) Clues to the cells of origin in retinoblastoma. Int Ophthalmol Clin 20:191–210
Virchow R (1864) Die krankhaften Geschwülste, vo. 2. Hirschwald, Berlin
Weinberg RA (1991) Tumor suppressor genes. Science 254:1138–1146
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Yuge, K., Nakajima, M., Miki, H. et al. Immunohistochemical features of the human retina and retinoblastoma. Vichows Archiv A Pathol Anat 426, 571–575 (1995). https://doi.org/10.1007/BF00192111
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DOI: https://doi.org/10.1007/BF00192111