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Differential epitope expression of Ly-48 (mouse leukosialin)

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Abstract

Ly-48 is a major sialoglycoprotei expressed on the surface of variety of mouse hematopoietic cells that exhibits many characteristics isoforms and may function in signal transduction and cell adhension. Ly-48 is recognized by the 3E8-specific monoclonal antibody (mAb) and it has been suggested that it is the same antigen recognized by another mAb known as S7. In this report, we demonstrate definitively by transfection of a Ly-48 cDNA that S7 and two previously uncharacterized mAbs, S11 and S15, recognize the same antigen as teh 3E8-specific mAb. However, 2-D gel immunoblot analyses demonstrate the complex nature of Ly-48. Although all four mAbs react similarly with lysates from the M-45 B-cell myeloma line, 2-D immunobot analyses of the EL-4 T-cell line reveal three distinct patterns of reactivity. Further, while transfection of Ly-48 into the K562 erythroleukemic cell line conferred reactivity to all four mAbs, transfection of the Ly-48 cDNA into the nonhematopoietic cell line, Line 1, conferred reactivity only to the S11 and S15 mAbs. Thus, the Line 1 transfectants suggest the importance of posttranslational modifications in the expression of the 3E8 and S7 epitopes. Interestingly , developing fetal liver cells show the same pattern of differential Ly-48-specific mAb reactivity. The developing early fetal liver cells are reactive with S11 and S15 but are negative, to very weakly, reactive with the 3E8-and S7-specific mAbs. These results show that Ly-48 epitopes can be expressed independently on cell lines in vitro and are differentially expressed on healthy cells in vivo.

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Authors and Affiliations

  1. Cancer Center Immunology Division and Department of Microbiology and Immunology, University of Rochester Medical Center, Box 704, 14642, Rochester, NY, USA

    Clare M. Baecher-Allan, Karen S. Dorfman, Richard K. Barth & John G. Frelinger

  2. Department of Pathology, University of Iowa Hospitals and Clinics, 52242, Iowa City, IA, USA

    John D. Kemp

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  1. Clare M. Baecher-Allan
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  5. John G. Frelinger
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Baecher-Allan, C.M., Kemp, J.D., Dorfman, K.S. et al. Differential epitope expression of Ly-48 (mouse leukosialin). Immunogenetics 37, 183–192 (1993). https://doi.org/10.1007/BF00191883

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