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Heat-inducible rice hsp82 and hsp70 are not always co-regulated

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Abstract

We have characterized several heat-shock-induced genes in rice (Oryza sativa L.) and compared their expression under a variety of conditions. Three of these genes, which are analogs of the hsp82/90 family, lie within a cloned 18-kilobase (kb) region of the genome. The middle member of this cluster, designated hsp82B, has been fully sequenced. The gene uses a promoter containing six putative heat-shock elements as well as several unusual sequence motifs including a stretch of 11 thymidines alternating with 11 adenosines. The mRNA for this gene reaches its highest relative level of expression within 120 min after plants are shifted to 42° C; no other conditions induce this gene. By contrast, we found that during heat stress the expression of hsp70 correlates well with increases in internal ion concentrations, and can also be induced by excess salt or ethanol at normal growth temperatures. These results appear to indicate that whereas hsp70 is induced by all stresses that lead to protein denaturation — including heat stress — HSP82 mRNA accumulates only upon heat stress.

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Abbreviations

HSF:

heat shock factor

HSP:

heat shock protein

kb:

kilobase

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Author notes

  1. Rudy Dekeyser

    Present address: Instituut ter bevordering van het Wetenschappelijk Onderzoek en Technologie in Viaanderen, B-1000, Brussels, Belgium

  2. Bart Claes

    Present address: European Patent Office, D-80298, München, Germany

  3. Allan B. Caplan

    Present address: Department of Bacteriology and Biochemistry, University of Idaho, 83843, Moscow, ID, USA

Authors and Affiliations

  1. Laboratorium voor Genetica, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium

    Frank Van Breusegem, Rudy Dekeyser, Ana Beatriz Garcia, Bart Claes, Jan Gielen, Marc Van Montagu & Allan B. Caplan

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  1. Frank Van Breusegem
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Additional information

The authors thank R. Villarroel (Laboratorium voor Genetica, Universiteit Gent, Gent, Belgium) for help with the sequencing, both Dr. Van Der Straeten and D. Van den Broeck (Laboratorium voor Genetica, Universiteit Gent, Gent, Belgium) for critical reading of the manuscript, Martine De Cock for typing it, and Karel Spruyt and Vera Vermaercke for drawings and photographs. This work was supported by grants from the Belgian Programme on Interuniversity Poles of Attraction (Prime Minister's Office, Science Policy Programming #38), the Commission of the European Communities TS2-0053-B (GDF), and the Rockefeller Foundation (FR 86058 #59). A.B.G. is indebted to Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil; RHAE proc. no. 260015188.1) for a predoctoral fellowship.

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Van Breusegem, F., Dekeyser, R., Garcia, A.B. et al. Heat-inducible rice hsp82 and hsp70 are not always co-regulated. Planta 193, 57–66 (1994). https://doi.org/10.1007/BF00191607

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