Abstract
The effect of day/night temperature regimes on stem elongation and on the content of endogenous gibberellins (GAs) in vegetatively propagated plants of Campanula isophylla cv. Hvit have been studied. Compared with a constant temperature regime at 18°C (18/18°C), stem and internode elongation was enhanced significantly by a combination of high day/low night temperature (21/15°C) and inhibited by an opposite regime (15/21°C). Gibberellins A1, A19, A44, A53, and A97 were identified as endogenous components in Campanula. (GA97 was earlier referred to as 2β-OH-GA53.) Quantitative analysis of the endogenous GAs indicates that temperature regimes that stimulate elongation growth are accompanied by an increase in the level of GA1, GA19, and GA44. On the other hand, in plants grown under conditions that reduced stem elongation growth, there was an increased level of GA97.
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Abbreviations
- DIF:
-
difference between day temperature and night temperature
- GA:
-
gibberellin
- HPLC:
-
high performance liquid chromatography
- GC-MS:
-
gas chromatography-mass chromatography
- SPE:
-
solid phase extraction
- TMS:
-
trimethylsilyl
- MSTFA:
-
N-methyl-N-TMS-trifluoroacetamide
- KRI:
-
Kovats retention index
- SIM:
-
selected ion monitoring
- D2:
-
deuterated
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Jensen, E., Eilertsen, S., Ernsten, A. et al. Thermoperiodic control of stem elongation and endogenous gibberellins in Campanula isophylla . J Plant Growth Regul 15, 167–171 (1996). https://doi.org/10.1007/BF00190580
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DOI: https://doi.org/10.1007/BF00190580