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Through-bond correlation of imino and aromatic resonances in 13C-,15N-labeled RNA via heteronuclear TOCSY

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Summary

Novel HCCNH TOCSY NMR experiments are presented that provide unambiguous assignment of the exchangeable imino proton resonances by intranucleotide through-bond connectivities to the (assigned) nonexchangeable purine H8 and pyrimidine H6 protons in uniformly 15N-, 13C-labeled RNA oligonucleotides. The HCCNH TOCSY experiments can be arranged as a two-dimensional experiment, correlating solely GH8/UH6 and GH1/UH3 proton resonances (HCCNH), of as three-dimensional experiments, in which additional chemical shift labeling either by GN1/UN3 (HCCNH) or by GC8/UC6 (HCCNH) chemical shifts is introduced. The utility of these experiments for the assignment of relatively large RNA oligonucleotides is demonstrated for two different RNA molecules.

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Authors and Affiliations

  1. Department of Physical and Theoretical Chemistry, Masaryk University, CZ-611 37, Brno, Czech Republic

    Vladimír Sklenář

  2. Institute of Scientific Instruments, Academy of Sciences of the Czech Republic, CZ-612 64, Brno, Czech Republic

    Vladimír Sklenář

  3. Department of Chemistry and Biochemistry, University of California, P.O. Box 951569, 90095-1569, Los Angeles, CA, USA

    Thorsten Dieckmann, Samuel E. Butcher & Juli Feigon

  4. Molecular Biology Institute, University of California, P.O. Box 951569, 90095-1569, Los Angeles, CA, USA

    Thorsten Dieckmann, Samuel E. Butcher & Juli Feigon

Authors
  1. Vladimír Sklenář
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  2. Thorsten Dieckmann
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  3. Samuel E. Butcher
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  4. Juli Feigon
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Sklenář, V., Dieckmann, T., Butcher, S.E. et al. Through-bond correlation of imino and aromatic resonances in 13C-,15N-labeled RNA via heteronuclear TOCSY. J Biomol NMR 7, 83–87 (1996). https://doi.org/10.1007/BF00190460

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  • DOI: https://doi.org/10.1007/BF00190460

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