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Detection of bacterial β-galactosidase activity in individual Saccharomyces cerevisiae cells by flow cytometry

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Summary

A fluorogenic substrate and trapping reagent have been used to detect β-galactosidase activity expressed in S. cerevisiae from an Escherichia coli lacZ gene segment encoded on a recombinant plasmid. The measurement may be made in less than 1 msec using a flow cytometer, facilitating rapid experimental characterization of plasmid instability.

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Authors and Affiliations

  1. Division of Chemistry and Chemical Engineering California, Institute of Technology, 91125, Pasadena, California, USA

    Friedrich Srienc, Judith L. Campbell & James E. Bailey

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  1. Friedrich Srienc
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  2. Judith L. Campbell
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  3. James E. Bailey
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Srienc, F., Campbell, J.L. & Bailey, J.E. Detection of bacterial β-galactosidase activity in individual Saccharomyces cerevisiae cells by flow cytometry. Biotechnol Lett 5, 43–48 (1983). https://doi.org/10.1007/BF00189963

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  • DOI: https://doi.org/10.1007/BF00189963

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