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Usage of TCRAV and TCRBV gene families in human fetal and adult TCR rearrangements

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Abstract

We have investigated fetal and adult T-cell receptor (TCR) A and B V-gene repertoires both by fluorescence-activated cell sorter (FACS) analysis with the avialable TCR V region-specific mAbs and by the polymerase chain reaction (PRC) with TRC V gene family-specific oligonucleotides. Among the low number of CD3+ T cells, most of the TRC V region tested for could be detected by FACS analysis in liver, bone marrow, and spleen derived from a 14-week-old fetus and two 15-weeks-old fetuses. Similarly, the PCR analysis showed that the majority of the TCRAV and TCRBV families were expressed in the peripheral organs of the 13-week-old fetus, although an apparent absence of particular TCR V families was found in liver and bone marrow. This was most probably the consequence of the low number of CD3+ T cells in these organs. In 17-week-old week-old fetal thymi the level of expression of some TCRAV and TCRBV gene families, in particular those that contain single member, was lower compared to post-partum thymi and adult peripheral blood mononuclear cells. The combined data of FACS and PCR analysis demonstrate that TCR genes belonging to the majority of TCR V gene families can be used in TCR α and β chain rearrngements during early human fetal life. Our data also suggest that the expression levels of some of the single member TCR V gene families may be influenced by the development stage.

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Authors and Affiliations

  1. Department of Immunohematology and Bloodbank, Leiden University Hospital, P.O Box 9600, 2300 RC, Laiden, The Netherlands

    Frank M. Raaphorst, Jeroen van Bergen & Peter J. van den Elsen

  2. Department of Pediatrics, Leiden University Hospital, P.O. Box 9600, 2300 RC, Laiden, The Netherlands

    Frank M. Raaphorst, Renée Langlios van den Bergh, Maarten J. D. van Tol & Jaak M. Vossen

  3. Department of Cytochemistry and Cytometry Sylvius Laboratories, Leiden University, 2333 AA, Wassenaarseweg 72, Leuden, The Netherlands

    Maarten van der Keur

  4. Department of Pediatrics, Subdivision of clinical Genetics, Erasmus University/Sophia Children's Hospital, Dr. Molenwaterplein 60, 3015 GJ, Rotterdam, he Netherlands

    Ronald de Krijger & Jan Bruining

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  1. Frank M. Raaphorst
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  2. Jeroen van Bergen
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Raaphorst, F.M., van Bergen, J., van den Bergh, R.L. et al. Usage of TCRAV and TCRBV gene families in human fetal and adult TCR rearrangements. Immunogenetics 39, 343–350 (1994). https://doi.org/10.1007/BF00189231

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  • DOI: https://doi.org/10.1007/BF00189231

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