Summary
Two winter wheat genotypes (‘Diószegi 200’ and ‘Mv 15’) were compared for their in vitro androgenic capacity. On average, the induction frequency of embryogenic structures was 71.7% in ‘Diószegi 200’ and only 4.3% in ‘Mv 15’. The haploid induction ability of the two genotypes differed considerably, with ‘Diószegi 200’ being much higher. The difference in the in vitro inductability of the microspores may result from genetic differences which are manifested in the survival rate of the microspores during the culture period and their adaptability to in vitro conditions. Special DNA fluorochrornes were suitable for studying the different pathways of in vitro androgenesis. Our data indicate that the repeated equal divisions of the microspore nucleus might lead to pollen embryo formation, and subsequent divisions of the vegetative portion of the pollen grain after the first asymmetric microspore mitosis can result in pollen callus formation.
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References
Barnabás B, Fransz PF, Schel JNH (1987) Ultrastructural studies on pollen embryogenesis in maize (Zea mays L.). Plant Cell Rep 6:212–215
Chuang CC, Ouyang TW, Chia H, Chou SM, Ching CK (1978) A set of potato media for wheat anther culture. In: Proceedings of the Symposium on Plant Tissue Culture. Science Press, Beijing, pp 51–56
Coleman AW, Goff LJ (1985) Application of fluorochromes to pollen biology. I. Mithramycin and 4,6-diamidino-2-phenylindole as vital stains and for quantitation of nuclear DNA. Stain Technol 60:145–154
Dunwell JM (1978) Division and differentiation in cultured pollen. In: Thorpe TA (ed) Frontiers of plant tissue culture. University Calgary Press, Calgary, pp 103–112
Guha S, Maheshwari SC (1964) In vitro production of embryos from anther of Datura. Nature 204:497
Guha S, Maheshwari SC (1966) Cell division and differentiation of embryos in the pollen grains of Datura in vitro. Nature 212:97–98
He DG, Ouyang JW (1985) Observation of androgenesis in cultured wheat anthers at meiosis, tetrad, early uninucleate and trinucleate stage. Acta Bot Sin 27:469–475
Heberle-Bors E (1985) In vitro haploid formation from pollen: a critical review. Theor Appl Genet 71:361–374
Henry Y, DeBuyser J, Gueneguo T, Ory C (1984) Wheat microspore embryogenesis during in vitro anther culture. Theor Appl Genet 67:439–442
Hough T, Berhardt P, Knox RB, Williams EG (1985) Application of fluorochromes to pollen biology. II. The DNA probes ethidium bromide and Hoechst-33258 in conjunction with the callose specific anilin blue fluorochrome. Stain Technol 60:155–162
Huang B (1986) Ultrastructural aspects of pollen embryogenesis in Hordeum, Triticum and Paeonia, In: Han H, Hongyuan Y(eds) Haploids of higher plants in vitro. Springer, Berlin Heidelberg New York, pp 91–118
Idzikowska K, Ponitka A, Mlodzianowski F (1982) Pollen dimorphism and androgenesis in Hordeum vulgare. Acta Soc Bot Pol 51:153–156
Kyo M, Harada H (1986) Studies on conditions for cell divisions and embryogenesis in isolated pollen culture of Nicotiana rustica. Plant Physiol 79:90–94
Miao S, Kuo C, Kwei Y, Ku S, Lu W, Wang Y, Chen N, Wu M, Hang L (1978) Induction of pollen plants of maize and observation on their progeny. In: Proceedings of the Symposium on Plant Tissue Culture. Science Press, Beijing, pp 23–33
Ouyang TW, Chuang CC, Tseng CC (1973) Induction of pollen plants from anthers of Triticum aestivum L. cultured in vitro. Sci Sin 16:79–95
Pace GM, Reed NJ, Ho IC, Fahey JW (1987) Anther culture of maize and the visualization of embryogenic microspores by fluorescent microscopy. Theor Appl Genet 73:863–869
Pan JL, Gao GM (1980) To repeat the effects of hormones on the microspore developments of wheat in vitro anther culture. Acta Bot Sin 22:305–310
Raghavan V (1976) Role of the generative cell in androgenesis in henbane. Science 191:388–389
Raghavan V (1986) Embryogenesis in angiosperms. Cambridge University Press, Cambridge, pp 152–189
Sun C (1978) Androgenesis of cereal crops. In: Proceedings of the Symposium on Plant Tissue Culture. Science Press, Beijing, pp 117–123
Sun C, Chu C, Li H (1983) Electron microscope observation of microspore division of wheat in vitro. Acta Bot Sin 25:295–300
Sunderland N, Dunwell JM (1977) Anther and pollen culture. In: Street HF(ed) Plant tissue and cell culture, 2nd edn. University of California Press, Berkeley, pp 223–265
Sunderland N, Evans LJ (1980) Multicellular pollen formation in cultured barley anthers. II. The A, B, and C pathways. J Exp Bot 31:501–514
Vasil IK (1980) Androgenetic haploids. Int Rev Cytol [Suppl] 11A:195–223
Vergne P, Dilvallee I, Dumas C (1987) Rapid assessment of microspore and pollen development stage in wheat and maize using DAPI and membrane permeabilization. Stain Technol 62:299–304
Zheng J, Ouyang J (1980) The early androgenesis in in vitro wheat anthers under ordinary and low temperature. Acta Genet Sin 7:165–175
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Szakács, É., Barnabás, B. Cytological aspects of in vitro androgenesis in wheat (Triticum aestivum L.) using fluorescent microscopy. Sexual Plant Reprod 1, 217–222 (1988). https://doi.org/10.1007/BF00189155
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DOI: https://doi.org/10.1007/BF00189155