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Evidence for at least three transcribed BoLA class I loci

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Abstract

Serological data identify a single major histocompatibility complex (MHC) class I locus in cattle. Molecular data, however, demonstrate the presence of at least two cattle MHC (BoLA) class I loci. To investigate the number of transcribed BoLA class I genes, we amplified cattle cDNA by using a single MHC class I-specific primer that hybridized to a conserved region of exon 4 and a non-specific 3′ primer. Six BoLA class I cDNAs have been cloned and sequenced from a Bos taurus bull heterozygous for BoLA class I serological antigens, demonstrating the presence of a minimum of three loci. Sequence comparisons suggested that one of these cDNAs may be an unexpressed allele or the product of a nonclassical locus.

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Author notes

  1. Theodore L. Garber

    Present address: Wisconsin Regional Primate Research Center, University of Wisconsin, 53715, Madison, WI, USA

Authors and Affiliations

  1. Department of Veterinary Pathology, Texas A & M University, 77843, College Station, TX, USA

    Theodore L. Garber & Joe W. Templeton

  2. Department of Biology and Institute of Molecular Genetics, Pennsylvania State University, 16802, University Park, PA, USA

    Austin L. Hughes

  3. Wisconsin Regional Primate Research Center, University of Wisconsin, 53715, WI, Madison, USA

    David I. Watkins

Authors
  1. Theodore L. Garber
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  2. Austin L. Hughes
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  3. David I. Watkins
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  4. Joe W. Templeton
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Additional information

The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers U01186 and U01187.

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Garber, T.L., Hughes, A.L., Watkins, D.I. et al. Evidence for at least three transcribed BoLA class I loci. Immunogenetics 39, 257–265 (1994). https://doi.org/10.1007/BF00188788

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  • DOI: https://doi.org/10.1007/BF00188788

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