Skip to main content
Log in

Ultrastructural localization of actin in the cell body of rat spinal ganglion neurons

  • Original Article
  • Published:
Anatomy and Embryology Aims and scope Submit manuscript

Abstract

We used phalloidin staining and immunocytochemistry at the light and electron microscope level to determine the localization of actin in the cell bodies of rat spinal ganglion neurons. The results show that actin is mostly concentrated along the periphery of the neuronal perikaryon, including the perikaryal projections. This localization places actin in a strategic position to be influenced by incoming signals and to produce mechanical tensions able to shape the perikaryal surface.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Hay M, De Boni U (1991) Chromatin motion in neuronal interphase nuclei: changes induced by disruption of intermediate filaments. Cell Motil Cytoskeleton 18:63–75

    Google Scholar 

  • Kuczmarski ER, Rosenbaum JL (1979) Studies on the organization and localization of actin and myosin in neurons. J Cell Biol 80:356–371

    Google Scholar 

  • Lankford KL, Letourneau PC (1991) Roles of actin filaments and three second-messenger systems in short-term regulation of chick dorsal root ganglion neurite outgrowth. Cell Motil Cytoskeleton 20:7–29

    Google Scholar 

  • Letourneau PC (1981) Immunocytochemical evidence for colocalization in neurite growth cones of actin and myosin and their relationship to cell-substratum adhesions. Dev Biol 85:113–122

    Google Scholar 

  • Letourneau PC (1989) Nerve cell shape. In: Stein WD, Bronner F (eds) Cell shape: determinants, regulation, and regulatory role. Academic Press, San Diego, pp 247–289

    Google Scholar 

  • Letourneau PC, Ressler AH (1983) Differences in the organization of actin in the growth cones compared with the neurites of cultured neurons from chick embryos. J Cell Biol 97:963–973

    Google Scholar 

  • Nagele RG, Kosciuk MC, Hunter ET, Bush KT, Lee H (1988) Immunoelectron microscopic localization of actin in neurites of cultured embryonic chick dorsal root ganglia: actin is a component of granular, microtubule-associated crossbridges. Brain Res 474:279–286

    Google Scholar 

  • Obata K, Inoue H (1982) Development of microspikes and neurites in cultured dorsal root ganglion cells. Neurosci Lett 30:1–5

    Google Scholar 

  • Pannese E, Gioia M, Carandente O, Ventura R (1983) A quantitative electron microscope study of the perikaryal projections of sensory ganglion neurons. I. Cat and rabbit. J Comp Neurol 214:239–250

    Google Scholar 

  • Pannese E, Bianchi R, Gioia M, Ventura R (1985) A quantitative electron microscope study of the perikaryal projections of sensory ganglion neurons. II. Gecko and lizard. J Comp Neurol 240:212–218

    Google Scholar 

  • Pannese E, Ledda M, Conte V, Procacci P, Matsuda S (1990a) Scanning electron-microscope observations of the perikaryal projections of rabbit spinal ganglion neurons after enzymatic removal of connective tissue and satellite cells. Cell Tissue Res 260:167–173

    Google Scholar 

  • Pannese E, Ledda M, Conte V, Procacci P (1990b) The perikaryal projections of rabbit spinal ganglion neurons. A comparison of thin section reconstructions and scanning microscopy views. Anat Embryol 181:427–432

    Google Scholar 

  • Pannese E, Rigamonti L, Ledda M, Arcidiacono G (1994) Perikaryal projections of spinal ganglion neurons: quantitative differences between membrane domains in contact with different microenvironments. J Anat 185:497–502

    Google Scholar 

  • Pannese E, Ledda M, Conte V, Rigamonti L, Procacci P (1995) On the influence of the perineuronal microenvironment on the outgrowth of perikaryal projections of spinal ganglion neurons. J Submicrosc Cytol Pathol 27:303–308

    Google Scholar 

  • Shaw G, Osborn M, Weber K (1981) Arrangement of neurofilaments, microtubules and microfilament-associated proteins in cultured dorsal root ganglia cells. Eur J Cell Biol 24:20–27

    Google Scholar 

  • Spooner BS, Holladay CR (1981) Distribution of tubulin and actin in neurites and growth cones of differentiating nerve cells. Cell Motil 1:167–178

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pannese, E., Procacci, P. & Ledda, M. Ultrastructural localization of actin in the cell body of rat spinal ganglion neurons. Anat Embryol 194, 527–531 (1996). https://doi.org/10.1007/BF00187466

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00187466

Key words

Navigation