Abstract
The nucleotide sequences of 38 T-cell receptor (Tcr) β-chain cDNA clones which were isolated from a cDNA library (2 × 106 plaques) constructed from bovine peripheral blood lymphocytes were determined. Of 38 cDNA clones, 22 were rearranged and contained the functional variable (V) gene segments. These clones were tentatively divided into nine Tcrb-V gene families which correspond to the human Tcrb-V family. Among them, a Tcrb-V12 gene segment was isolated from 9 out of 22 clones, suggesting that this Tcrb-V family was expressed in the bovine peripheral blood lymphocytes. Two different constant (C) geen segments were found, and both C regions were composed of 178 amino residues. The amino acid sequences of bovine Tcrb-C regions are approximately 80%–82%, 78%, and 78% similar to those from human, mouse, and rabbit, respectively. To estimate Tcrb-V-associated restriction fragment length polymorphisms (RFLPs), Southern blot analysis was performed using liver DNAs from four bovine breeds, Holstein, Angus, Hereford, and Japanese Black. However, no significant difference was observed among genomic DNAs of Tcrb-V loci from these four breeds.
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Angiolillo, A. L., Lamoyi, E., Bernstein, K. E., and Mage, R. G.: Identification of genes for the constant region of rabbit T-cell receptor β chains. Proc Natl Acad Sci USA 82: 4498–4502, 1985
Barth, R. K., Kim, B. S., Lan, N. C., Humkapiller, T., Sobieck, N., Winoto, A., Gershenfeld, H., Okada, C., Hamsburg, D., Weissman, I. L., and Hood, L.: The murine T-cell receptor uses a limited repertoire of expressed Vβ gene segments. Nature 316: 517–523, 1985
Behlke, M. A., Spinella, D. G., Chou, H. S., Sha, W., Hartl, D. L., and Loh, D. Y.: T-cell receptor β-chain expression: dependence on relatively few variable region genes. Science 229: 566–570, 1985
Chien, Y.-H., Becker, D. M., Lindsten, T., Okamura, M., Cohen, D. I., and Davis, M. M.: A third type of murine T-cell receptor gene. Nature 312: 31–35, 1984a
Chien, Y.-H., Gascoigne, N. R. J., Kavalver, J., Lee, N. E., and Davis, M. M.: Somatic recombination in a murine T-cell receptor gene. Nature 309: 322–326, 1984b
Chien, Y.-H., Iwashima, M., Kaplan, K. B., Elliott, J. F., and Davis, M. M.: A new T-cell receptor gene located within the alpha locus and expressed early in T-cell differentiation. Nature 327: 677–682, 1987
Clark, S. P., Yoshikai, Y., Siu, G., Taylor, S., Hood, L., and Mak, T. W.: Identification of a diversity segment of human T-cell receptor β-chain, and comparison with the analogous murine element. Nature 311: 387–389, 1984
Concannon, P., Pickering, L. A., Kung, P., and Hood, L.: Diversity and structure of human T-cell receptor β-chain variable region genes. Proc Natl Acad Sci USA 83: 6598–6602, 1986
Feinberg, A. P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 266–267, 1983
Flung, F., Pelicci, P.-G., Bonetti, F., Knowles, D. M., II, and Dalla-Favera, R.: T-cell receptor gene rearrangements as markers of lineage and clonality in T-cell neoplasms. Proc Natl Acad Sci USA 82: 3460–3464, 1985
Harday, A. C., Diamond, D. J., Tanigawa, G., Heilig, J. S., Folsom, V., Saito, H., and Tonegawa, S.: Unusual organization and diversity of T-cell receptor α-chain genes. Nature 316: 828–836, 1985
Haskins, K., Hannum, C., White, J., Roehm, N., Kubo, R., Kappler, J., and Marrack, P.: The antigen-specific, major histocompatibility complex-restricted receptor on T cells. VI. Antibody to a receptor allotype. J Exp Med 160: 452–471, 1984
Hedrick, S. M., Nielsen, E. A., Kavaler, J., Cohen, D. I., and Davis, M. M.: Sequence relationship between putative T-cell receptor polypeptides and immunoglobulins. Nature 308: 153–158, 1984
Ikuta, K., Ogura, T., Shimizu, A., and Honjo, T.: Low frequency of somatic mutation in β-chain variable region genes of human T-cell receptors. Proc Natl Acad Sci USA 82: 7701–7705, 1985
Ishiguro, N., Tanaka, A., and Shinagawa, M.: Sequence analysis of bovine T-cell receptor α chain. Immunogenetics 31: 57–60, 1990
Jones, N., Leiden, J., Dialynas, D., Fraser, D., Clabby, M., Kishimoto, T., and Strominger, J. L.: Partial primary structure of the alpha and beta chains of human tumor T-cell receptors. Science 227: 311–314, 1985
Kimura, N., Toyonaga, B., Yoshikai, Y., Triebel, F., Debra, P., Minden, M. D., and Mak, T. W.: Sequences and diversity of human T-cell receptor beta-chain variable region genes. J Exp Med 164: 739–750, 1986
Kimura, N., Toyonaga, B., Yoshikai, Y., Due, R.-P., and Mak, T. W.: Sequences and repertoire of the human T cell receptor α and β chain variable region genes in thymocytes. Eur J Immunol 17: 375–383, 1987
Kotzin, B. L., Virgenia, B. L., and Edward, P.: A large deletion within the T-cell receptor beta-chain gene complex in New Zealand white mice. Science 229: 167–171, 1985
Leidein, J. M. and Strominger, J. L.: Generation of diversity of the β chain of the human T-lymphocyte receptor for antigen. Proc Natl Acad Sci USA 83: 4456–4460, 1986
Malissen, M., Minard, K., Mjolsness, S., Kroneberg, M., Goverman, J., Hunkapiller, T., Prystowsky, M. B., Yoshikai, Y., Fitch, F., Mak, T. W., and Hood, L.: Mouse T cell antigen receptor: structure and organization of constant and joining gene segments encoding the β polypeptide. Cell 37: 1101–1110, 1984
Maniatis, T., Fritsch, E. F., and Sambrook, J.: Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, 1982
Marche, P. N. and Kindt, T. J.: A variable region gene subfamily encoding T cell receptor β-chains is selectively conserved among mammals. J Immunol 137: 1729–1734, 1986
Meuer, S. C., Acuto, O., Hussey, R. E., Hodgdon, J. C., Fitzgerald, K. A., Schlossman, S. F., and Reinherz, E. L.: Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor. Nature 303: 808–810, 1983
Minden, M. D., Toyonaga, B., Ha, K., Yanagi, Y., Chin, B., Gelfand, E., and Mak, T. W.: Somatic rearrangement of T-cell antigen receptor gene in human T-cell malignancies. Proc Natl Acad Sci USA 82: 1224–1227, 1985
Saito, H., Kranz, D. M., Takagaki, Y., Hayday, A. C., Eisen, H. N., and Tonegawa, S.: Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequences. Nature 309: 757–762, 1984a
Saito, H., Kranz, D. M., Takagaki, Y., Hayday, A. C., Eisen, H. N., and Tonegawa, S.: A third rearranged and expressed gene in a clone of cytotoxic T lymphocytes. Nature 312: 36–40, 1984b
Sanger, F., Nicklen, S., and Coulson, A. R.: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467, 1977
Shevach, E. M. and Rosenthal, A. S.: Function of macrophages in antigen recognition by guinea pig T lymphocytes. II. Role of the macrophage in the regulation of genetic control of the immune response. J Exp Med 138: 1213–1229, 1973
Siu, G., Kronenberg, M., Strauss, E., Haars, R., Mak, T. W., and Hood, L.: The structure, rearrangement and expression of Dβ gene segments of the murine T-cell antigen receptor. Nature 311: 344–345, 1984
Takashima, I., Olson, C., Driscoll, D. M., and Baumgartener, L. E.: B-lymphocytes and T-lymphocytes in three types of bovine lymphosarcoma. J Natl Cancer Inst 59: 1205–1209, 1977
Toyonaga, B., Yoshikai, Y., Vadasz, V., Chin, B., and Mak, T. W.: Organization and sequences of the diversity, joining, and constant region genes of the human T-cell receptor β chain. Proc Natl Acad Sci USA 82: 8624–8628, 1985
Watson, C. J. and Jackson, J. F.: An alternative procedure for the synthesis of double-stranded cDNA for cloning in phage and plasmid vectors. In D. M. Glover (ed.): DNA Cloning, Vol 1, A Practical Approach, pp. 79–88, Oxford, Washington DC, 1985
Williams, C. B. and Gutman, G. A.: T cell receptor β-chain genes in the rat. Availability and pattern of utilization of V gene segments differs from that in the mouse. J Immunol 142: 1027–1035, 1989
Yanagi, Y., Yoshikai, Y., Leggett, K., Clark, S. P., Aleksander, I., and Mak, T. W.: A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains. Nature 308: 145–149, 1984
Yoshikai, Y., Yanagi, Y., Suciu-Foca, N., and Mak, T. W.: Presence of T-cell receptor mRNA in functionally distinct T cells and elevation during intrathymic differentiation. Nature 310: 506–508, 1984
Yoshikai, Y., Clark, S. P., Taylor, S., Sohn, U., Wilson, B. I., Minden, M. D., and Mak, T. W.: Organization and sequences of the variable, joining and constant region genes of the human T-cell receptor α-chain. Nature 316: 837–840, 1985
Zinkernagel, R. M. and Doherty, P. C.: H-2 compatibility requirement for T-cell-mediated lysis of target cells infected with lymphocytic choriomeningitis virus: different cytotoxic T-cell specificities are associated with structures from coded for in H-2K or H-2D. J Exp Med 141: 1427–1436, 1975
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The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers D90121-40.
Address correspondence and offprint requests to: N. Ishiguro.
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Tanaka, A., Ishiguro, N. & Shinagawa, M. Sequence and diversity of bovine T-cell receptor β-chain genes. Immunogenetics 32, 263–271 (1990). https://doi.org/10.1007/BF00187097
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DOI: https://doi.org/10.1007/BF00187097