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Carbonyldiimidazole activation of a rayon/polyester cloth for covalent immobilization of proteins

  • Immobilization Techniques
  • Published:
Biotechnology Techniques

Summary

A non-woven rayon/polyester cloth was treated with NaOH and activated with 1,1-carbonyldiimidazole. The activated cloth was washed and used without drying to immobilize bovine serum albumin. Drying of the cloth markedly decreased protein immobilization. The advantages of using the activated cloth for industrial affinity chromatography were discussed.

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References

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Authors and Affiliations

  1. Department of Biology, Carleton University, K1S 5B6, Ottawa, Canada

    Jay R. Howlett & Hiroshi Yamazaki

  2. Institute for Biological Sciences, National Research Council, K1A 0R6, Ottawa, Canada

    David W. Armstrong

Authors
  1. Jay R. Howlett
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  2. David W. Armstrong
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  3. Hiroshi Yamazaki
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Howlett, J.R., Armstrong, D.W. & Yamazaki, H. Carbonyldiimidazole activation of a rayon/polyester cloth for covalent immobilization of proteins. Biotechnol Tech 5, 395–400 (1991). https://doi.org/10.1007/BF00185022

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  • DOI: https://doi.org/10.1007/BF00185022

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