Summary
A bacterium belonging to the Bacillus firmus/lentus-complex and capable of growth on native potato starch was isolated from sludge of a pilot plant unit for potato-starch production. Utilization of a crude enzyme preparation obtained from the culture fluid after growth of the microorganism on native starch, resulted in complete degradation of native starch granules from potato, maize and wheat at a temperature of 37°C. Glucose was found as a major product. Production of maltose, maltotriose and maltotetraose was also observed. Native-starch-degrading activity (NSDA) could be selectively adsorbed on potato-starch granules, whereas soluble-starch-degrading activity (SSDA) remained mainly in solution. The use of such a starch-adsorbed enzyme preparation on native starch resulted in a completely changed product pattern. An increase in oligosaccharides concomitant with less glucose formation was observed. An increased conversion of soluble starch to maltopentaose was possible with this starch-adsorbed enzyme preparation. It is concluded that NSDA comes from α-amylase(s) and SSDA from glucoamylase(s) and/or α-glucosidase(s). Cultivation of B. firmus/lentus on glucose, maltose, or soluble starch resulted in substantially smaller quantities of (native) starch-degrading activity.
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References
Abe J, Bergmann W, Obata K, Hizukuri S (1988) Production of the raw-starch digestion amylase of Aspergillus sp. K-27. Appl Microbiol Biotechnol 27:447–450
Brunt K (1982) Rapid separation of linear and cyclic glucooligosaccharides on a cation exchange resin using Ca2+-EDTA solution as eluent. J Chromatogr 246:145–151
Buchanan RE, Gibbons NE (ed) (1974) Bergey's manual of determinative bacteriology, 8th edn Williams and Wilkins, Baltimore
Buranakari L, Ito K, Izaki K, Takahashi H (1988) Purification and characterization of a raw starch-digestive amylase from non-sulfur purple photosynthetic bacterium. Enzyme Microb Technol 10:173–179
Dische Z (1962) Color reactions of hexoses. In: Whistler RL, Wolfrom ML, BeMiller JN, Shafizadeh F (ed) Methods in carbohydrate chemistry, Vol I. Academic Press, New York, pp 490–491
Fogarty WM (1983) Microbial amylases. In: Fogarty WM (ed) Microbial enzymes and biotechnology. Applied Science Publishers, London, pp 1–93
Hayashida S (1975) Selective submerged production of three types of glucoamylases by a black koji mold. Agric Biol Chem 39:2093–2099
Hayashida S, Teramoto I, Inoue T (1988) Production and characteristics of raw-potato-starch digesting α-amylase from Bacillus subtilis 65. Appl Environ Microbiol 54:466–472
Kelly CT, Moriarty M, Fogarty WM (1983) α-Glucosidase of Bacillus firmus. Biotechnol Lett 5:289–294
Matsuoka H, Koba Y, Ueda S (1982) Alcoholic fermentation of sweet potato without cooking. J Ferment Technol 60:599–602
Meers JL (1972) The regulation of α-amylase production in Bacillus licheniformis. Anthonie van Leeuwenhoek 38:585–590
Monma M, Kagei N, Kainuma K (1989) Raw starch digestion by Chalara paradoxa immobilized in calcium alginate. Starch 41:355–357
Nelson N (1952) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153:376–380
Sata H, Taniguchi H, Maruyama Y (1987) Regulation of amylase synthesis in Bacillus circulans F-2. Agric Biol Chem 51:1521–1527
Sawada M, Kurusawa K, Sasaki H, Takao S (1988) Method for direct saccharification of raw starch using enzyme produced by a basidiomycete belonging to the genus Corticium. US patent no. 4727026
Tanaka T, Ishimoto E, Shimomura Y, Taniguchi M (1987) Purification and some properties of raw starch-binding amylase of Clostridium butyricum T7 isolated from mesophilic methane sludge. Agric Biol Chem 51:399–405
Tani Y, Fuji A, Nishise H (1988) Production of raw cassava starch-digestive glucoamylase by a 2-deoxyglucose resistant mutant of Rhizopus sp. J Ferment Technol 66:545–551
Taniguchi H, Odashima F, Igarishi M, Maruyama Y, Nakamurav M (1982) Characterization of a potato starch-digesting bacterium and its production of amylase. Agric Biol Chem 46:2107–2115
Ueda S, Zenin CT, Monteiro DA, Park YK (1981) Production of ethanol from raw cassava starch by a nonconventional fermentation method. Biotechnol Bioeng 23:291–299
Yoshigi N, Chicano T, Kamimura M (1985) Purification and properties of an amylase from Bacillus cereus NY-14. Agric Biol Chem 49:3369–3376
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Wijbenga, DJ., Beldman, G., Veen, A. et al. Production of native-starch-degrading enzymes by a Bacillus firmus/lentus strain. Appl Microbiol Biotechnol 35, 180–184 (1991). https://doi.org/10.1007/BF00184683
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DOI: https://doi.org/10.1007/BF00184683