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Colorimetric assay for lignin peroxidase activity determination using methylene blue as substrate

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Biotechnology Techniques

Summary

A colorimetric method for determination of lignin peroxidase activity has been developed which is based on the demethylation of methylene blue. The use of this dye shows the practical advantage of using a wavelength in the visible region of spectrum. The method can be efficiently used for the enzyme quantification as its sensitivity is close to the veratryl alcohol assay.

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Authors and Affiliations

  1. Instituto de Química, Universidade Federal do Rio de Janeiro, RJ, Brasil

    Denise Bello Magalhães & Elba Bon

  2. Faculdade de Engenharia Química de Lorena, SP, Brasil

    Maria Eleonora Andrade de Carvalho

  3. Escola de Química, Universidade Federal do Rio de Janeiro, RJ, Brasil

    Júlio Silva Araújo Neto

  4. Instituto de Química, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, Maracanã, CEP 20550-000, Rio de Janeiro, RJ, Brasil

    Sérgio Hélio Kling

Authors
  1. Denise Bello Magalhães
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  2. Maria Eleonora Andrade de Carvalho
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  3. Elba Bon
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  4. Júlio Silva Araújo Neto
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  5. Sérgio Hélio Kling
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Magalhães, D.B., de Carvalho, M.E.A., Bon, E. et al. Colorimetric assay for lignin peroxidase activity determination using methylene blue as substrate. Biotechnol Tech 10, 273–276 (1996). https://doi.org/10.1007/BF00184028

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  • DOI: https://doi.org/10.1007/BF00184028

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