Summary
The effect of simple cold storage in Euro-Collins solution on prolonging successful preservation time was examined on an established rat limb transplant model. Limbs amputated at mid-thigh level were simply immersed in the solution and stored in a refrigerator at 4°C. After varying periods of cold storage, the limb was orthotopically grafted to another isogeneic rat using microsurgical techniques. The survival rates for 12, 24, 48 and 72 hours' storage were 100%, 90%, 67% and 50% respectively, and the longest time of successful preservation achieved was 78 hours. Survival rates were greatly decreased when amputated limbs were initially perfused with Euro-Collins solution and immersed in the same solution. In a third set of experiments, the limbs were preserved by a conventional method of cold storage in air, and the survival rates were decreased even further. Evaluation by measuring postoperative paw swelling and microscopic examination of the grafts further confirmed the effectiveness of the simple immersion method without perfusion.
Résumé
Nous avons étudié, sur la patte de rat, choisie comme modèle de transplant, l'effet du simple stockage au froid dans la solution Euro-Collins sur la prolongation du temps de conservation. Les pattes, amputées à mi-cuisse, ont été simplement plongées dans la solution, puis conservées dans un réfrigérateur à la température de 4°C. Après des durées variables de stockage au froid les pattes ont été greffées sur d'autres rats isogéniques, de façon micro-chirurgicale. Le taux de survie des transplants après 12, 24, 48 et 72 heures de conservation fut respectivement de 100%, 90%, 67% et 50%, et la plus longue durée de conservation efficace fut de 78 heures. Les taux de survie ont diminué de façon importante lorsque les transplants étaient d'abord perfusés avec la solution Euro-Collins puis immergés dans cette même solution. Dans un troisième groupe d'expériences, les pattes amputées furent conservées de façon traditionnelle par stockage au froid à l'air, et les taux de survie des greffes diminuèrent encore plus. L'évaluation par la mesure du gonflement de la patte greffée ainsi que l'examen microscopique ont confirmé l'efficacité de la simple immersion sans perfusion.
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Arai, K., Hotokebuchi, T., Miyahara, H. et al. Successful long-term storage of rat limbs. International Orthopaedics 17, 389–396 (1993). https://doi.org/10.1007/BF00180460
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DOI: https://doi.org/10.1007/BF00180460