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Induction of the fed pattern of human exocrine pancreatic secretion by nutrients: role of cholecystokinin and neurotensin

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Summary

The aim of the present study was to assess the role of cholecystokinin and neurotensin in converting the cyclical interdigestive pattern of pancreatic secretion into the non-cyclical fed pattern. Six healthy male volunteers were studied on 4 separate days. During each experiment a mixed liquid meal or solutions of individual nutrients were perfused intraduodenally for 180 min at 2 ml/min. The mixed meal contained 4.3 g glucose, 2.0 g fractionated soya oil, and 1.7 g casein hydrolysate per 100 ml, which delivered a caloric load of 0.9 kcal/min into the duodenum. The isocaloric and isotonic solutions of individual nutrients contained 44.5 g glucose, 17.8 g fractionated soya oil, or 44.5 g hydrolysed serum bovine albumin per liter and delivered 0.36 kcal/min into the duodenum. Duodenal aspirates and blood samples were collected at regular intervals for determination of pancreatic enzyme outputs and plasma levels of cholecystokinin and neurotensin, respectively. The mixed meal converted the cyclical interdigestive secretory pattern into the noncyclical fed pattern whereas none of the three individual nutrients abolished the interdigestive pattern. Not only the mixed meal but also lipid and protein perfusion consistently stimulated cholecystokinin release. Integrated incremental cholecystokinin release amounted to 32.3±9.9 pg/ml × 180 min with the mixed meal, 23.2±6.5 with lipid perfusion (P< 0.05 versus mixed meal) and 13.4±3.8 with protein perfusion (P<0.05 versus mixed meal). The carbohydrate solution did not significantly release cholecystokinin. None of the duodenal perfusates raised neurotensin plasma levels. We conclude that (a) intraduodenal delivery of a mixed meal at 0.9 kcal/min converts the interdigestive pattern of pancreatic secretion, (b) cholecystokinin but not neurotensin is involved in converting this pattern in response to low-caloric meals, and (c) a threshold amount of CCK release must be exceeded to convert the secretory pattern.

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Abbreviations

CCK:

cholecystokinin

PEG:

polyethylene glycol

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Authors and Affiliations

  1. Abteilung Gastroenterologie der Universität, Marburg

    M. Katschinski, C. Dippel, M. Reinshagen, J. Schirra & R. Arnold

  2. Chirurgische Klinik der Universität, Göttingen

    R. Nustede

  3. Departement Gastroenterologie des Universitätsspitals, Basel

    C. Beglinger

  4. Abteilung Innere Medizin I der Universität, Ulm

    G. Adler

  5. Zentrum für Innere Medizin, Baldingerstrasse, W-3550, Marburg, Germany

    M. Katschinski

Authors
  1. M. Katschinski
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  2. C. Dippel
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  3. M. Reinshagen
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  4. J. Schirra
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  5. R. Arnold
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  6. R. Nustede
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  7. C. Beglinger
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  8. G. Adler
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Katschinski, M., Dippel, C., Reinshagen, M. et al. Induction of the fed pattern of human exocrine pancreatic secretion by nutrients: role of cholecystokinin and neurotensin. Clin Investig 70, 902–908 (1992). https://doi.org/10.1007/BF00180436

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  • DOI: https://doi.org/10.1007/BF00180436

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