Summary
Light and electron microscopic as well as immunohistochemical investigations were performed on the basement membrane (BM) of the nasal mucosa from the inferior nasal conchae of 20 patients, aged 15–50 years. Two of the patients served as controls. Clinical disorders requiring the turbinectomies were hyperplastic nasal inferior conchae (17 patients) and immobile cilia syndrome (1 patient). In all cases light microscopy demonstrated a 10–15 μm thick homogeneous BM underneath an intact epithelium. Electron microscopic findings revealed a typical subepithelial basal lamina (BL). The remaining parts of the BM consisted of single, isolated 25-mm-thick collagenous fibrils. Only a few cells and small unmyelinated nerves occurred in this layer. Immunohistochemical investigations showed BL components (collagen type IV, laminin, nidogen and heparan sulfate proteoglycan) directly underneath the epithelium. Collagen types I, III, V, and VI could also be demonstrated immunohistochemically in the remaining parts of the BM. Collagen type VII was allocated to the anchor filaments beneath the BL. This special BM is presumed to contribute to mechanical stabilization of the epithelium. The significance of the BL and the subepithelial connective tissue for the behavior of the epithelium — e.g. direction of differentiation - are discussed.
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Barrach HJ, Grundmann K, Hinz N, Felies A (1980) Immunofluorescent microscopic investigations of intercellular substances during limb development. In: Merker H-J, Nan H, Neubert D (eds) Teratology of the limbs. de Gruyter, Berlin, pp 273–293
Barrach HJ, Grundmann K, Kwasigroch TE, Hinz N, Felies A (1981) Comparison of the differentiation of muscle and connective tissue of mouse limb buds in culture and in vivo. In: Neubert D, Merker H-J (eds) Culture technqiues. de Gruyter, Berlin, pp 135–159
Bruckner-Tuderman L, Mitsuhashi Y, Schnyder VW, Bruckner P (1989) Anchoring fibrils and type VII collagen are absent from skin in severe recessive dystrophic epidermolysis bullosa. J Invest Dermatol 93:3–9
Chew EC (1980) On tannic acid fixation and staining. Acta Anat 128:156–159
Csato W, Merker H-J (1983) Production and formation of the basement membrane in embryonic tissue of the mouse. Cell Tissue Res 228:85–95
Futaesaku Y, Mizuhira V, Nakamura H (1972) The new fixation method using tannic acid for electron microscopy and some observations of biological specimens. Proc Int Congr Histochem Cytochem 4:155
Goodman SL, Risse G, von der Mark K (1980) The E8 subfragment of laminin promotes locomotion of myoblasts over extracellular matrix. J Cell Biol 109:799
Gosslau B, Barrach HJ (1979) Enzyme-linked immunosorbent microassay from quantification of specific antibodies to collagen type I, II, III. J Immunol Methods 29:71–77
Grover A, Edwards SA, Bourdon M, Adamson ED (1987) Proteoglycan-19, laminin and collagen type IV production is correlated with the levels of mRNA in F9 cell aggregates differentiating in the presence or absence of cyclic AMP. Differentiation 36:138
Guo M, Grinnell F (1989) Basement membrane and human epidermal differentiation in vitro. J Invest Dermatol 93:372
Hassel JR, Robey PG, Barrach HJ (1980) Isolation of heparan sulfate-containing proteoglycan from basement membrane. Proc Natl Acad Sci USA 77:4494–4498
Karst W, Merker H-J (1988) The differentiation behaviour of MDCK cells grown on matrix components and in collagen gels. Cell Differ 22:211–224
Keene DR, Sakai LY, Lumstrum GP, Burgeson RE (1987) Type VII collagen forms an extended network of anchoring fibrils. J Cell Biol 104:611–621
Kittelberger-Ewert R, Hinz N, Oechsner I, Schröter-Kermani C, Barrach HJ (1988) Production of specificity of antibodies against the central region of type II collagen. Immunol Invest 17:49–61
Kleinman HK, Murray JC, McGoodwin EB, Martin GR (1978) Connective tissue structure: cell binding to collagen. J Invest Dermatol 71:9–11
Kohnert-Stavenhagen E, Zimmermann B (1980) Changes in the surface coat of mesenchymal cells of mouse limb buds after enzymatic cell isolation. J Exp Morphol Embryol 59:145–155
Leigh IM, Purkis PE, Bruckner-Tuderman L (1987) LH7.2 monoclonal antibody detects type VII collagen in the basement membrane of ectodermally derived epithelia including skin. Epithelia 1:17–29
Lilja S, Barrach HJ (1983) Normally sulphated and highly sulphated GAG affecting fibrillogenesis of type I and type 11 collagen in vitro. Exp Pathol 23: 173–181
Merker H-J (1987) Production and formation of basal lamina. In: Wolff JR (ed) Mesenchymal epithelial interaction in the neural development. NATO ASI, vol H5. Springer, Berlin Heidelberg New York, pp 54–64
Merker H-J (1993) The morphology of the basement membrane. Microsc Res Tech (in press)
Merker H-J, Zimmermann B, Barrach HJ, Grundmann K, Ebel H (1980) Simulation of steps of limb skeletogenesis in vitro. In: Merker H-J, Nau H, Neubert D (eds) Teratology of the limbs. de Gruyter, Berlin, pp 137–151
Merker H-J, Krüger U, Zimmermann B (1981) Simulation of limb bud skeletogenesis in vitro. In: Neubert D, Merker H-J (eds) Culture techniques. de Gruyter, Berlin, pp 119–133
Merker H-J, Bremer D, Barrach HJ, Gossrau R (1987) The basement membrane of the persisting maternal blood vessels in the placenta of Callithrix jacchus. Anat Embryol 176:87–97
Miekka SI, Ingham KC, Menache D (1982) Rapid methods for isolation of human plasma fibronectin. Thromb Res 27:1–14
Öcalan M, Goodman SL, Kühl U, Hauschka SD, van der Mark K (1988) Laminin alters cell shape and stimulates motility and proliferation of murine skeletal myoblasts. Dev Biol 125:158
Paulsson M, Aumailley M, Deutzmann R, Timpl R, Beck K, Engel J (1987) Extraction with chelating agents and structural characterization. Laminin-nidogen complex. Eur J Biochem 166:11–19
Rannels SR, Yarnell JA, Fisher CS, Fabisiak JP, Rannels DE (1987) Role of laminin in maintenance of type II pneumocyte morphology and function. Am J Physiol 253:C835-C845
Sage H, Bornstein P (1979) Characterization of a novel collagen chain in human placenta and its relation to AB collagen. Biochemistry 18:3815–3823
Sage H, Woodbury RG, Bornstein P (1979) Structural studies on human type IV collagen. J Biol Chem 254:9893–9900
Sakai IY, Keene DR, Morris NP, Burgeson RE (1986) Type VII collagen is a major structural component of anchoring fibrils. J Cell Biol 103:1577–1586
Sannes PL, Katsuyama T, Spicer SS (1978) Tannic acid — metal salt sequences for light and electron microscopic localization of complex carbohydrates. J Histochem Cytochem 26: 55–61
Schröter-Kermani C, Hinz N, Risse P, Zimmermann B, Merker H-J (1991) The extracellular matrix in cartilage organoid culture: biochemical, immunomorphological and electron microscopic studies. Matrix 11: 428–441
Singley CT, Solursh H (1980) The use of tannic acid for the ultrastructural visualizaton of hyaluronic acid. Histochemistry 65:93–102
Smith BD, Niles R (1980) Characterization of collagen synthesized by normal and chemically transformed rat liver epithelial cell lines. Biochemistry 19:1820–1825
Sugrue SP, Hay ED (1986) The identification of extracellular matrix (ECM) binding sites on the basal surface of embryonic corneal epithelium and the effect of ECM binding on epithelial collagen production. J Cell Biol 102:1907–1916
Timpl R, Rohde H, Robey PG, Rennard SI, Foidart J-M, Martin GR (1979) Laminin — a glycoprotein from basement membranes. J Biol Chem 254:9933–9937
Trelstad RL, Catanese VM, Rubin DF (1976) Collagen fractionation: separation of native type I, II and III by differential precipitation. Ann Biochem 71:114–118
Wujek JP, Haleem-Smith H, Yamada Y, Lipsky R, Lan YT, Freese E (1990) Evidence that the Bλ chain of laminin is responsible for the neurite outgrowth promoting activity of astrocyte extracellular matrix. Dev Brain Res 55:237–248
Xu D, Schröter-Kermani C, Hinz N, Merker H-J (1989) Connective tissue of the livers of newborn and adult marmosets (Callithrix jacchus). Histol Histopathol 4:479–492
Xu D, Schröter-Kermani C, Hinz N, Merker H-J (1990) Immunofluorescence microscopic localization of collagen type IV and VI, laminin, and nidogen in the livers of Callithrix jacchus during pre- and postnatal development. Acta Anat 138: 212–219
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Agha-Mir-Salim, P., Rauhut, O. & Merker, H.J. Electron and fluorescence microscopic investigations on composition and structure of the epithelial basement membrane of the human inferior nasal concha. Eur Arch Otorhinolaryngol 250, 401–407 (1993). https://doi.org/10.1007/BF00180386
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DOI: https://doi.org/10.1007/BF00180386