Summary
Feeding glucose in response to pH changes was compared to batch trials and glucose estimation by gas data. The cell density was similar for all cases (~8 g/l dry wt). Glucose concentration was estimated to be ~0.006 g/l. The fed-batch by pH strategy had no detectable plasmid loss whereas fed-batch by estimate trials had 99% plasmid retention per generation (78% after 25 generations) and batch trials had only 95% (28% after 25 generations). Protein expression occurred as a consequence of the pH strategy and was not constitutive.
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Frude, M.J., Read, A. & Kennedy, L. Induction of recombinant protein production by pH stress: A novel glucose feeding strategy. Biotechnol Lett 15, 797–802 (1993). https://doi.org/10.1007/BF00180144
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DOI: https://doi.org/10.1007/BF00180144