Abstract
We have analyzed the brain distribution of the rat 5-HT4 receptor mRNA. A receptor specific probe was used for in situ hybridization of rat brain sections. Abundant expression of the 5-HT4 receptor mRNA was observed in the olfactory system, striatum, medial habenula and the hippocampal formation, while faint or no specific signals could be detected in most other areas of the brain. Several brain areas which display strong ligand binding do not contain mRNA, suggesting an axonal localization of the 5-HT4 receptor.
This is a preview of subscription content, log in via an institution to check access.
References
Bockaert J, Fozard JR, Dumuis A, Clarke DE (1992) The 5-HT4 receptor: a place in the sun. Trends Pharmacol Sci 13:141–145
Brown AM, Young TJ, Patch TL, Cheung CW, Kaumann AJ, Gaster L, King FD (1993) [125I]-SB 207710, a potent, selective radioligand for 5-HT4 receptors. Br J Pharmacol 110:10
Ford APDW, Clarke DE (1993) The 5-HT4 receptor. Med Res Revs 13:633–662
Gerald C, Adham N, Hung-Teh K, Olsen MA, Laz TM, Schlechter LE, Bard JA, Vaysse PJ-J, Hartig PR, Branchek TA, Weinshank RL (1995) The 5-HT4 receptor: molecular cloning and pharmacological characterization of two splice variants. EMBO J 14:2806–2815
Grossmann CJ, Kilpatrick GJ, Bunce KT (1993) Development of a radioligand binding assay for 5-HT4 receptors in guinea-pig and rat brain. Br J Pharmacol 109:618–624
Hoyer D, Clarke DE, Fozard JR, Hartig PR, Martin GR, Mylecharane EJ, Saxena PR, Humphrey PPA (1994) VII International Union of Pharmacology classification of receptors for 5-hydroxytryptamine (serotonin). Pharmacological Rev 46:157–203
Jakeman LB, To ZP, Eglen RM, Wong EHF, Bonhaus DW (1994) Quantitative autoradiography of 5-HT4 receptors in brains of three species using structurally distinct radioligands, [3H]GR113808 and [3H]BIMU-1. Neuropharmacology 33:1027–1038
McCabe JT, Pfaff DW (1989) Gene probes. In: Conn PM (eds) Academic Press, San Diego, pp 7.19–7.22
Mengod G, Nguyen H, Le H, Waeber C, Lübbert H, Palacios JM (1990) The distribution and cellular localization of the serotonin 1C receptor mRNA in the rodent brain examined by in situ hybridization histochemistry. Comparison with receptor binding distribution. Neuroscience 35:577–591
Patel S, Roberts J, Moorman J, Reavill C (1995) Localization of serotonin-4 receptors in the striatonigral pathway in rat brain. Neuroscience 69:1159–1167
Paxinos G, Watson C (1986) The rat brain in stereotaxic coordinates. Academic Press, San Diego
Ullmer C, Schmuck K, Kalkmann HO, Lüdbbert H (1995) Expression of serotonin receptor rnRNAs in blood vessels. FEBS Lett 370:215–221
Vilaro MT, Wiederholt KH, Palacios JM, Mengod G (1992) Muscarinic M2 receptor mRNA expression and receptor binding in cholinergic and non-cholinergic cells in the rat brain: a correlation study using in situ hybridization histochemistry and receptor autoradiography. Neuroscience 47:367–393
Waeber C, Sebben M, Nieoullon A, Bockaert J, Dumuis A (1994) Regional distribution and ontogeny of 5-HT4 binding sites in rodent brain. Neuropharmacology 35:527–541
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ullmer, C., Engels, P., Abdel'Al, S. et al. Distribution of 5-HT4 receptor mRNA in the rat brain. Naunyn-Schmiedeberg's Arch Pharmacol 354, 210–212 (1996). https://doi.org/10.1007/BF00178723
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00178723