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Comparison of the optimal culture conditions for cell growth and tissue plasminogen activator production by human embryo lung cells on microcarriers

  • Biotechnology
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Abstract

Optimization of culture conditions such as the dissolved O2 (DO) concentration, temperature and pH was attempted regarding both cell growth and the production of tissue plasminogen activator (TPA) in a microcarrier cell culture of human embryo lung cells. The growth rate was suppressed at a DO concentration below 30% saturation. From the pH range 7.2–7.6, both the specific growth rate and maximal cell concentration decreased. At a lower temperature than 37°C, although both the specific growth rate and the maximal cell concentration decreased, the cell concentration was maintained for a longer time during the production period, high TPA productivity being maintained. As the optimal conditions for culture growth, a DO concentration of 30% saturation or over, temperature of 37°C and pH of 7.4 are recommended. However, for TPA production after cell culture growth, the DO concentration should be in the range 20–30% O2 saturation, and the temperature and pH should be lowered to 33°C and 6.8, respectively.

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Authors and Affiliations

  1. Life Science Research Laboratories, Asahi Chemical Industry Company Ltd., 2-1, Samejima, 416, Fuji, Shizuoka, Japan

    M. Takagi & K. Ueda

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  1. M. Takagi
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  2. K. Ueda
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Takagi, M., Ueda, K. Comparison of the optimal culture conditions for cell growth and tissue plasminogen activator production by human embryo lung cells on microcarriers. Appl Microbiol Biotechnol 41, 565–570 (1994). https://doi.org/10.1007/BF00178490

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  • DOI: https://doi.org/10.1007/BF00178490

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