Abstract
The only avian major histocompatibility complex (Mhc) genes thus far identified are from species of the relatively small order of Galliformes, while by far the largest order of Passeriformes (songbirds), containing some 60% of extant bird species, has not been studied at all in this regard. The Galliformes emerged more than 55 million years (my) ago, the Passeriformes some 25 my ago. Because of the potential for the use of Mhc genes as markers in the study of songbird populations, an attempt was made to clone class II B genes of a passeriform species, the Bangalese finch Lonchura striata acuticauda. Using a set of primers designed on the basis of known sequences, a probe corresponding to part of exon II was obtained by the polymerase chain reaction. The probe was then used to screen a Bengalese finch cDNA library and to isolate and sequence two nearly full-length clones. The sequences reveal the presence of one presumably functional class II B locus in this bird species.
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References
All, S. and Ripley, S. D. Handbook of Birds of India and Pakistan, Vol 10, Oxford University Press, Bombay, 1974
Bourlet, Y., Béhar, G., Guillemot, F., Frechin, N., Billault, A., Chaussé, A. M., Zoorob, R., and Auffray, C. Isolation of chicken major histocompatibility complex class II (B-L) β chain sequences: comparison with mammalian β chains and expression in lymphoid organs. EMBO J 7: 1031–1039, 1988
Bucham, J. The Bengalese Finch, H. L. Iles, Kingswood, Bristol, 1976
Feduccia, A. Explosive evolution in tertiary birds and mammals. Science 267: 637–638, 1995
Feinberg, A. P. and Vogelstein, B. A. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137: 6–13, 1983
Giorda, R., Turco, E., and Trucco, M. Full-length β chain cDNAs of DQw9 and DQw8 molecules encode proteins that differ only at amino acid 57. Immunogenetics 33: 404–408, 1991
Goodwin, D. Estrilid Finches of the World. British Museum (Natural History). Comstock Publications Assoc., Cornell University Press, Ithaca, NY, 1982
Klein, J. and Figueroa, F. Evolution of the major histocompatibility complex. CRC Crit Rev Immunol 6: 295–386, 1986
Klein, J., Bontrop, R. E., Dawkins, R. L., Erlich, H. A., Gyllensten, U. B., Heise, E. R., Jones, P. P., Parham, P., Wakeland, E. K., and Watkins, D. I. Nomenclature for the major histocompatibility complexes of different species: a proposal. Immunogenetics 31: 217–219, 1990
Maniatis, T., Fritsch, E. F., and Sambrook, J. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, 1991
Ogawa, S., Nishimura, U., Awaji, M., Nozawa, S., Hirose, S., and Shirai, T. Nucleotide sequence analysis of MHC class II genes in autoimmune disease-prone (NZB × NZW) F1 mice. Immunogenetics 32: 63–67, 1990
Ono, H., Klein, D., Vincek, V., Figueroa, F., O'hUigin, C., Tichy, H., and Klein, J. Major histocompatibility complex class II genes of the zebrafish. Proc Natl Acad Sci USA 89: 11886–11890, 1992
Perrins, C. M. and Middleton, A. L. A. (eds.): The Encyclopedia of Birds, p. 422, Facts on File, New York, 1985
Pharr, G. T., Dodgson, J. B., and Bacon, L. D. Analysis of B-L beta chain gene expression in two chicken cDNA libraries. Immunogenetics 37: 381–385, 1993
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Erlich, H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491, 1988
Saitou, M. and Nei, M. The neighbor-joining method. A new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425, 1987
Sanger, F., Nicklen, S., and Coulson, A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467, 1977
Sato, K., Flajnik, M. F., Pasquier, L. D., Katagiri, M., and Kasahara, M. Evolution of the MHC: isolation of class II beta-chain cDNA clones from the amphibian Xenopus laevis. J Immunol 150: 2831–2843, 1992
Stapel, S. O., Leunissen, J. A. M., Versteeg, M., Wattel, J., and deJong, W. V. Ratites as oldest offshoot of avian stem-evidence from α-crystallin A sequences. Nature 311: 257–259, 1984
Wittzell, H., von Schantz, T., Zoorob, R., and Auffray, C. Molecular characterization of three Mhc class II β haplotypes in the ring-necked pheasant. Immunogenetics 39: 395–403, 1994
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Vincek, V., Klein, D., Graser, R.T. et al. Molecular cloning of major histocompatibility complex class II B gene cDNA from the Bengalese finch Lonchura striata . Immunogenetics 42, 262–267 (1995). https://doi.org/10.1007/BF00176443
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DOI: https://doi.org/10.1007/BF00176443