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References
Chomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 159–163, 1987
Fischer, S. G. and Lerman, L. S. Length-independent separation of DNA restriction fragments in two-dimensional gel electrophoresis. Cell 16: 191–200, 1979
Fischer, S. G., and Lerman, L. S. DNA fragments differing by single base pair substitutions are separated in denaturing gradient gels: correspondence with melting theory. Proc Natl Acad Sci USA 80: 1579–1583, 1983
Itoh, N., Yonehara, S., Ishii, A., Yonehara, M., Mizushima, S., Sameshima, M., Hase, A., Seto, Y., and Nagata, S. The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. Cell 66: 233–243, 1991
Myers, R. M., Lumelsky, N., Lerman, L. S., and Maniatis, T. Detection of single base substitutions in total genomic DNA. Nature 313: 495–498, 1985 a
Myers, R. M., Fischer, S. G., Maniatis, T., and Lerman, L. S. Modification of the melting properties of duplex DNA by attachment of a GC-rich DNA sequence as determined by denaturing gradient gel eletrophoresis. Nucleic Acids Res 13: 3111–3129, 1985 b
Myers, R. M., Fischer, S. G., Lerman, L. S., and Maniatis, T. Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis. Nucleic Acids Res 13: 3131–3145, 1985 c
Myers, R. M., Maniatis, T., and Lerman, L. S. Detection and localization of single base changes by denaturing gradient gel electrophoresis. Methods Enzymol 155: 501–527, 1987
Myers, R. M., Sheffield, V. C., and Cox, D. R. Mutation detection by PCR, GC-clamp and denaturing gradient gel electrophoresis. In H. A. Erlich (ed.): PCR Technology, pp. 71–78, Stockton, New York, 1989
Oehm, A., Behrmann, I., Falk, W., Pawlita, M., Maier, G., Klas, C., Li-Weber, M., Richards, S., Dhein, J., Trauth, B. C., Postingl H., and Krammer P. H. Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor family. J Biol Chem 267: 10709–10715, 1992
Sheffield, V. C., Cox, D. R., Lerman, L. S., and Myers, R. D. Attachment of a 40-base-pair G+C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proc Natl Acad Sci USA 86: 232–236, 1989
Trauth, B. C., Klas, C., Peters, A. M. J., Matzku, S., Moller, P., Falk, W., Debatin, K. M., and Krammer, P. H. Monoclonal antibody-mediated tumor regression by induction of apoptosis. Science 245: 301–305, 1989
Watanabe-Fukunaga, R., Brannan, C.I., Copeland, N. G., Jenkins, N. A., and Nagata, S. Lymphoproliferation disorder in mice explained by defects in Fas antigen that mediates apoptosis. Nature 356: 314–317, 1992 a
Watanabe-Fukunaga, R., Brannan, C. I., Itoh, N., Yonehara, S., Copeland, N. G., Jenkins, N. A., and Nagata, S. The cDNA structure, expression, and chromosomal assignment of the mouse Fas antigen. J Immunol 148: 1274–1279, 1992 b
Yonehara, S., Ishi, A., and Yonehara, M. A cell-killing monoclonal antibody (anti-Fas) to a cell surface antigen co-downregulated with the receptor of tumor necrosis factor. J Exp Med 169: 1747–1756, 1989
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Correspondence to: G. Ruberti.
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Fiucci, G., Ruberti, G. Detection of polymorphisms within the FAS cDNA gene sequence by GC-clamp denaturing gradient gel eletrophoresis. Immunogenetics 39, 437–439 (1994). https://doi.org/10.1007/BF00176163
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DOI: https://doi.org/10.1007/BF00176163