Skip to main content
SpringerLink
Log in

Protein tyrosine (de-) phosphorylation in head and neck squamous cell carcinoma

  • Original paper
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Protein phosphorylation plays an important role in signal transduction of both normal and neoplastic cells. Since increased protein tyrosine phosphorylation may be associated with malignant transformation, we studied the activities of protein tyrosine kinases (PTK) and protein tyrosine phosphatases (PTPase) in patients with various head and neck tumors. Furthermore, we determined the patterns of tyrosine phosphorylated protein (P-tyr) in tissues by western blotting. Enzyme activities were studied in tumor and histologically normal, non-tumorous tissues of 54 patients and in 11 controls. P-tyr patterns were determined in 3 patients and 2 controls. PTK and PTPase activities were greater in tumor tissues than in normal tissue of the cancer patients as well as controls. P-tyr levels in tumors were also higher than in normal tissues. Additionally, PTK activity in normal tissue of tumor patients was significantly higher than in normal tissue of the control group. The same trend was observed for the PTPase activity and P-tyr levels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bishop JM (1987) The molecular genetics of cancer. Science 235:305–311

    Google Scholar 

  2. Erp HE van (1992) Protein tyrosine kinases in human brain, oligodendrogliomas and astrocytomas. J Neurochem 58:554–561

    Google Scholar 

  3. Field JK, Spandidos DA (1990) The role of ras and myc oncogenes in human solid tumours and their relevance in diagnosis and prognosis (review). Anticancer Res 10:1–22

    Google Scholar 

  4. Fischer EH, Charbonneau H, Tonks NK (1991) Protein tyrosine phosphatases: a diverse family of intracellular and transmembrane enzymes. Science 253:401–406

    Google Scholar 

  5. Hennipman A, Oirschot BA van, Smits J, Rijksen G, Staal GEJ (1989) Tyrosine kinase activity in breast cancer, benign breast disease, and normal breast tissue. Cancer Res 49:516–521

    Google Scholar 

  6. Hermanek P, Sobiq LH (1987) TNM classification of malignant tumours. (UICC, International union against cancer) Springer, Berlin Heidelberg New York

    Google Scholar 

  7. Hokanson J (1991) Discussion/perspective of ‘Beyond the “new” TNM classification’. Arch Otolaryngol 117:371

    Google Scholar 

  8. Klarlund JK (1985) Transformation of cells by an inhibitor of phosphatases acting on phosphotyrosine in proteins. Cell 41:707–717

    Google Scholar 

  9. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature (Lond) 227:680–685

    Google Scholar 

  10. Liebow C, Reilly C, Serrano M, Schally AV (1989) Somatostatin analogues inhibit growth of pancreatic cancer by stimulating tyrosine phosphatase. Proc Natl Acad Sci USA 86:2003–2007

    Google Scholar 

  11. Ottenhoff-Kalf AE, Rijksen G, Beurden EACM van, SchipperKester G, Adriaansen-Slot SS; Hennipman A, Michels AA, Staal GEJ (1992) Characterization of protein tyrosine kinases from human breast cancer; involvement of the c-src oncogene product. Cancer Res 52:4773–4778

    Google Scholar 

  12. Rijksen G, Oirschot BA van, Staal GEJ (1991) Nonradioactive assays of phospho-tyrosine kinase activity using anti-phosphotyrosine antibodies. In: Hunter T, Sefton BM (eds) Protein phosphorylation, part A (Methods in Enzymology, vol 200). Academic Press, San Diego, pp 98–106

    Google Scholar 

  13. Rijksen G, Völler MCW, Zoelen EJJ van (1993) Orthovanadate both mimics and antagonizes the transforming growth factor β action on normal rat kidney cells. J Cell Physiol 154:393–401

    Google Scholar 

  14. Riviere A, Wilckens C, Loning T (1990) Expression of cerbB2 and c-myc in squamous epithelia and squamous cell carcinomas of the head and neck and the lower female genital tract. J Oral Pathol Med 19:408–413

    Google Scholar 

  15. Riviere A, Becker J, Loning T (1991) Comparative investigation of c-erbB2/neu expression in head and neck tumors and mammary cancer. Cancer 67:2142–2149

    Google Scholar 

  16. Rydell EL, Olofsson J, Hellem S, Axelsson KL (1991) Tyrosine kinase activities in normal and neoplastic epithelia tissue of the human upper aero-digestive tract. Second Messengers Phosphoproteins 13:217–229

    Google Scholar 

  17. Scambia G, Panici PB, Battaglia F, Ferrandina G, Almadori G, Paludetti G, Maurizi M, Mancuso S (1991) Receptors for epidermal growth factor and steroid hormones in primary laryngeal tumors. Cancer 67:1347–1351

    Google Scholar 

  18. Slamon DJ, Kernion JB de, Verma IM, Cline MJ (1984) Expression of cellular oncogenes in human malignancies. Science 224:256–262

    Google Scholar 

  19. Tonks NK, Charbonneau H (1989) Protein tyrosine phosphorylation and signal transduction. Trends Biochem Sci 14:497–500

    Google Scholar 

  20. Ullrich A, Schlessinger J (1990) Signal transduction by receptors with tyrosine kinase activity. Cell 61:203–212

    CAS  PubMed  Google Scholar 

  21. Verschuur H, Rijksen G, Schipper-Kester GPM, Slootweg PJ, Hordijk GJ, Staal GEJ (1993) Protein tyrosine kinase activity in laryngeal squamous cell carcinoma. Eur Arch Otorhinolaryngol 249:466–469

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Otorhinolaryngology, University Hospital Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands

    H. P. Verschuur & G. J. Hordijk

  2. Laboratory of Medical Enzymology, Department of Hematology, University Hospital Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands

    G. Rijksen, B. A. van Oirschot, G. P. M. Schipper-Kester & G. E. J. Staal

  3. Department of Pathology, University Hospital Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands

    P. J. Slootweg

Authors
  1. H. P. Verschuur
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Rijksen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. A. van Oirschot
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. P. M. Schipper-Kester
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. J. Slootweg
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. E. J. Staal
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. G. J. Hordijk
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Verschuur, H.P., Rijksen, G., van Oirschot, B.A. et al. Protein tyrosine (de-) phosphorylation in head and neck squamous cell carcinoma. Eur Arch Otorhinolaryngol 251, 12–16 (1994). https://doi.org/10.1007/BF00175951

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00175951

Key words

Search

Navigation