Summary
A novel serum-free medium for the cultivation of Vero cells on microcarriers was developed,which composed of the 1:1 mixture of Dubecco's Modified Eagle Medium: Nutrient Mixture F12, bovine serum albumin(BSA) or human serum albumin(HSA), epidermal growth factor(EGF), gelatin and Dbiotin. Both BSA and EGF were effective on cell growth, adhesion and spreading. Further addition of gelatin and biotin led to the enhanced cell adhesion and spreading without growth promoting activity. The serum-free medium was suitable for the cultivation of vero cells on several different microcarriers with cell density reached over 3×l06cells/ml.
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References
Hayter PM, Curling EMA, Baines AJ, Jenkins N, Salmon I, Strabge PG, Tong JM and Bull AT (1992). Biotechnol. Bioeng. 39:327–335.
Jäger V, Lehmann J and Friedl P.(1988). Cytotechnology 1:319–329.
Litwin J. (1992). Cytotechnology 10:169–174.
Mendiaz E, Mamounas M, Moffett J and Englesberg E. (1986). In Vitro Cell. Develop. Biol. 22:66–74.
Merten O-W and Litwin J. (1991). Cytotechnology 5:69–82.
Mosmann T. (1983).J. Immunol. Methods 65:55–63.
Sato GH. (1975). The role of serum in cell culture. In: Biochemical actions of hormones, G. Litwack, ed. Vol. 3, pp391–396, New York: Academic Press.
Van Wezel AL.(1973). Microcarrier cultures of animal cells, In: Tissue culture, methods and applications. P. Kruse and MK. Patterson, eds. pp372–377, New York: Academic Press.
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Zhaolie, C., Chengzu, X., Hong, L. et al. A novel serum-free medium for the cultivation of Vero cells on microcarriers. Biotechnol Tech 10, 449–452 (1996). https://doi.org/10.1007/BF00174231
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DOI: https://doi.org/10.1007/BF00174231