Abstract
Spore progeny from an industrial baker's yeast strain were mutagenized with UV and mutants resistant to 2-deoxyglucose isolated. One of these mutants (10a12–13) showed high levels of maltase (α-glucosidase) and external invertase, and assimilated maltose when growing under catabolite repression conditions. This mutant was not allelic to any of the catabolite repression mutants tested cat4, cat80, cid1, cyc8, hex2, hxk2 and tup1. Mutant 10a12–13 was crossed with appropriate strains to construct hybrids that were also able to assimilate maltose in the presence of glucose. These hybrids may be useful in fermentation processes where both glucose and maltose are present.
Similar content being viewed by others
References
AACC (1983) Cereal laboratory methods and updated addenda. AACC American Association of Cereal Chemists, St. Paul, Minn.
Barber S, Torner MJ, Martinez-Anaya MA, Benedito de Berber C (1989) Microflora of the sour dough of wheat flour bread IX. Biochemical characteristics and baking performance of wheat doughs elaborated with mixtures of pure microorganisms. Z Lebensm Unters Forsch 189:6–11
Beudeker RF, Dam HW van, Plaat JB ven der, Vellenga K (1990) Developments in baker's yeast production. In: Verachtert H, De Mot R (eds) Yeast: biotechnology and biocatalysis. Dekker, New York, p 103–146
Burrows S (1979) Baker's yeast. Econ Microbiol 4:31–64
Entian K-D, Barnett JA (1992) Regulation of the sugar utilization by Saccharomyces cerevisiae. Trends Biochem Sci 17:506–510
Evans IH (1990) Yeast strains for baking: recent developments. In: Spencer JFT, Spencer DM (eds) Yeast technology. Springer, Berlin Heidelbeg New York p 13–54
Gancedo JM (1992) Carbon catabolite repression in yeast. Eur J Biochem 206:297–313
Gascon S, Lampen JO (1968) Purification of the internal invertase of yeast. J Biol Chem 243:1573–1577
Guthrie C, Fink GR (1991) Guide to yeast genetics and molecular biology. Methods Enzymol 194:21–93
Lovgren T, Hautera P (1977) Maltose fermentation and leavening ability of baker's yeast. Eur J Appl Microbiol 4:37–43
Okada H, Halvorson HO (1964) Uptake of alpha-thioethyl-glucopyranoside by Saccharomyces cerevisiae. 1. The genetic control of facilitated diffusion and active transport. Biochim Biophys Acta 82:538–542
Oliver S (1991) Classical yeast biotechnology. In: Tuite MF, Oliver SG (eds) Saccharomyces. Biotechnology handbooks vol 4. Plenum Press, London, p 213–248
Sherman F, Fink GR, Hicks JB (1986) Methods in yeast genetics. Cold Spring Harbor Laboratories Cold Spring Harbor, N. Y.
Trumbly RJ (1992) Glucose repression in the yeast Saccharomyces cerevisiae. Mol Microbiol 6:15–21
Vanoni M, Solliti P, Goldenthal M, Marmur J (1989) Structure and regulation of the multigene family controlling maltose fermentation in budding yeast. Prog Nucleic Acid Res Mol Biol 37:281–322
Zimmermann FK, Scheel I (1977) Mutants of Saccharomyces cerevisiae resistant to carbon catabolite repression. Mol Gen Genet 154:75–82
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Randez-Gil, F., Sanz, P. Construction of industrial baker's yeast strains able to assimilate maltose under catabolite repression conditions. Appl Microbiol Biotechnol 42, 581–586 (1994). https://doi.org/10.1007/BF00173924
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00173924