Summary
We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(l-alpha-aminoadipyl)-l-cysteinyl-d-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region was cloned which partially restores antibiotic production, ACV synthetase and cyclase activities to this mutant. The recombinant plasmid exhibits a variable copy number in different transformants. Hybridization experiments indicate that sequences homologous to the cloned region are present in various β-lactam-producing Streptomyces spp. but absent in species which are not known to produce this class of antibiotics. Furthermore, the chromosomal copy of the cloned region lies in close proximity to a gene coding for the isopenicilin N synthase gene of the cephalosphorin pathway.
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Piret, J., Resendiz, B., Mahro, B. et al. Characterization and complementation of a cephalosporin-deficient mutant of Streptomyces clavuligerus NRRL 3585. Appl Microbiol Biotechnol 32, 560–567 (1990). https://doi.org/10.1007/BF00173728
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DOI: https://doi.org/10.1007/BF00173728