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Protection of the ischemic myocardium from reperfusion injury by prostaglandin E1 inhibition of ischemia-induced neutrophil activation

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Summary

This study investigates the action of intravenous PGE1 on myocardial reperfusion injury and the possible involvement of antineutrophil activities. Cats were subjected to 3 h of temporary ligation of the left anterior descending coronary artery, followed by 2 h of reperfusion. Animals were treated with PGE1 (5 μg/kg x min) or vehicle (saline solution), starting 0.5 h after coronary artery occlusion. Vehicle-treated cats exhibited a significant loss of cardiac creatine phosphokinase specific activity at 5 h, accompanied by a significant ischemia-induced rise in the ST segment of the ECG and development of a Q wave after starting reperfusion. All of these alterations were largely prevented by PGE1 treatment. PGE1 exerted some blood-pressurelowering activity at 5 h (P > 0.05) but did not reduce myocardial contractile force and oxygen consumption. PGE1 modestly antagonized ischemia-induced formation of platelet aggregates. However, PGE1 prevented the rise in peripheral white blood cell count during ischemia and reperfusion and inhibited the generation of reactive oxygen species (myeloperoxidase assay) from zymosan-stimulated whole blood ex vivo. The ratio of generation of reactive oxygen species/white blood count remained unchanged. It is concluded that PGE1 protects the ischemic myocardium from acute reperfusion injury and that this effect involves an action of the compound on neutrophils, probably by improved myocardial tissue preservation, resulting in reduced formation of chemotactic products and, consequently, less local neutrophil accumulation and release of noxious metabolites.

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Author notes

  1. Christoph Thiemermann

    Present address: William-Harvey-Research Institute, St. Bartholomew's Hospital Medical College, ECIM 6BQ, London, UK

Authors and Affiliations

  1. Institut für Pharmakologie, Universität Düsseldorf, Moorenstrasse 5, D-4000, Düsseldorf 1, Federal Republic of Germany

    Karsten Schrör, Christoph Thiemermann & Peter Ney

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  1. Karsten Schrör
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  2. Christoph Thiemermann
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  3. Peter Ney
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Additional information

Parts of these results have been presented to the 29th Spring meeting of the Deutsche Gesellschaft für Pharmakologie und Toxikologie, Mainz, 1988

Send offprint requests to K. Schrör at the above address

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Schrör, K., Thiemermann, C. & Ney, P. Protection of the ischemic myocardium from reperfusion injury by prostaglandin E1 inhibition of ischemia-induced neutrophil activation. Naunyn-Schmiedeberg's Arch Pharmacol 338, 268–274 (1988). https://doi.org/10.1007/BF00173399

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  • DOI: https://doi.org/10.1007/BF00173399

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