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Molecular genetic strain improvement for the overproduction of fungal proteins by Filamentous fungi

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Abstract

During the last 5 years, strain improvement by genetic engineering has been established as a good alternative for traditional methods of strain improvement such as mutagenesis and genetic recombination. Considerable success has been achieved in the overproduction of a variety of fungal proteins. However, from the available data a limitation at the level of transcription is evident. A more detailed analysis of this transcription limitation was carried out for the overproduction of glucoamylase in A. niger. This analysis revealed that both the site of integration of introduced gene copies and the available amount of trans-acting regulatory proteins were at the root of the observed limitation. Based on these results, approaches for the construction of a new generation of protein-overproducing strains are suggested.

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  1. J. C. Verdoes

    Present address: Wageningen Agricultural University, Division of Industrial Microbiology, Department of Food Science, PO Box 8129, 6700, EV Wageningen, The Netherlands

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  1. TNO Nutrition and Food Research Institute, PO Box 5815, 2280, HV Rijswijk, The Netherlands

    J. C. Verdoes, P. J. Punt & C. A. M. J. J. van den Hondel

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Verdoes, J.C., Punt, P.J. & van den Hondel, C.A.M.J.J. Molecular genetic strain improvement for the overproduction of fungal proteins by Filamentous fungi. Appl Microbiol Biotechnol 43, 195–205 (1995). https://doi.org/10.1007/BF00172812

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