Summary
The effect of limiting the available oxygen on the fatty acid profile of Apiotrichum curvatum ATCC 20509 during growth on sulphuric acid casein whey was studied. At oxygen uptake rates (OUR) lower than 7 mmol O2/l per hour, applied during the oil accumulating phase of the fermentation, a decrease in total unsaturated fatty acids was observed. It was possible to decrease the unsaturated fatty acids (oleate from 55% to 41% and linoleate from 9% to 3%) by limiting the OUR of the culture to <3 mmol O2/l per hour. However at this low OUR, a lower oil coefficient (a measure of the efficiency of lactose substrate conversion to oil) was recorded. Furthermore the fermentation time was increased. An OUR of 5 mmol O2/l per hour appeared to be the limit below which adverse effects on oil yields and increased fermentation times occurred. At this OUR, the accumulated oil contained ≃45% oleate and ≃5% linoleate. These effects were demonstrated in a 20-l air-lift fermentor and confirmed in a scaled down 500-l industrial type bubble column fermentor.
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References
Babij T, Moss FJ, Ralph BJ (1969) Effects of oxygen and glucose levels on lipid composition of yeast Candida utilis grown in continuous culture. Biotechnol Bioeng 11:593–603
Brown CM, Rose AH (1969) Fatty acid composition of Candida utilis as affected by growth temperature and dissolved oxygen tension. J Bacteriol 99:371–378
Choi SY, Ryu DDY, Rhee JS (1982) Production of microbial lipid: effects of growth rate and oxygen on lipid synthesis and fatty acid composition of Rhodotorula gracilis. Biotechnol Bioeng 24:1165–1172
Davies RJ (1988) Yeast oil from cheese whey — process development. In: Moreton RS (ed) Single cell oil. Longman, UK, pp 99–145
Hall MJ, Ratledge C (1977) Lipid accumulation in an oleaginous yeast (Candida 107) growing on glucose under various conditions in a one- and two-stage continuous culture. Appl Environ Microbiol 33:577–584
Moreton RS (1985) Modification of fatty acid composition of lipid accumulating yeasts with cyclopropene fatty acid desaturase inhibitors. Appl Microbiol Biotechnol 22:41–45
Noguchi Y, Karre M, Iwamoto H (1982) Studies on lipid production by yeasts. Fatty acid composition of lipid from glucose and fatty acid esters by Rhodotorula and Candida. Yukagaku 31:431–437
Ratledge C (1982) Microbial oils and fats — an assessment of their commerical potential. Prog Ind Microbiol 16:119–206
Rattray JBM, Schibeci A, Kidby DK (1975) Lipids of yeasts. Bacteriol Rev 39:197–231
Tatsumi C, Hashimoto Y, Terashima M, Matsuo T (1977) Method for producing cocoa butter substitute. US patent no. 4032405
Verwoert II, Ykema A, Valkenburg JAC, Verbree EC, Nijkamp HJJ, Smit H (1989) Modified fatty acid compositions in lipids of intraspecific sphaeroplast fusion products of the oleaginous yeast Apiotrichum curvatum. In: Lipid production in the oleaginous yeast Apiotrichum curvatum. PhD Thesis (Ykema, A) Vrije University of Amsterdam, The Netherlands
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Davies, R.J., Holdsworth, J.E. & Reader, S.L. The effect of low oxygen uptake rate on the fatty acid profile of the oleaginous yeast Apiotrichum curvatum . Appl Microbiol Biotechnol 33, 569–573 (1990). https://doi.org/10.1007/BF00172553
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DOI: https://doi.org/10.1007/BF00172553