Summary
Stable transformation of suspension-cultured cells of tobacco with plasmid DNA (pCaMV-NEO) harbouring the neomycin phosphotransferase II (npt-II) gene was achieved using a previously described gas-pressure-driven particle acceleration device. The cells were bombarded with DNA-coated gold particles accelerated by the device, and callus containing the introduced gene was selected in the presence of geneticin disulphate. The geneticin-resistant callus exhibited npt-II enzyme activity, and Southern analysis confirmed the stable integration of the foreign gene into the tobacco genome. Transformants were obtained at a rate of more than 1.9 × 10−4.
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Iida, A., Morikawa, H. & Yamada, Y. Stable transformation of cultured tobacco cells by DNA-coated gold particles accelerated by gas-pressure-driven particle gun. Appl Microbiol Biotechnol 33, 560–563 (1990). https://doi.org/10.1007/BF00172551
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DOI: https://doi.org/10.1007/BF00172551