Summary
Addition of isopropyl β-D-thiogalactopyranoside (IPTG) to a strain of Escherichia coli with one lac promoter in its chromosome causes reduction in synthesis rate for a set of protiens. One of these proteins, designated H35, is a prominent cellular protein present only during exponential growth. Reduction of H35 synthesis is transient and delayed following an IPTG pulse. Cellular response to an IPTG pulse during exponential growth shares several features with a heat shock response. Significant increases in the specific growth rate of cells in both amino-acid-supplemented minimal medium and complex medium were observed for some IPTG concentrations relative to IPTG-free cultures. Other IPTG concentrations caused a reduction in specific growth rate.
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Kosinski, M.J., Rinas, U. & Bailey, J.E. Isopropyl-β-d-thiogalactopyranoside influences the metabolism of Escherichia coli . Appl Microbiol Biotechnol 36, 782–784 (1992). https://doi.org/10.1007/BF00172194
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DOI: https://doi.org/10.1007/BF00172194