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Major histocompatibility complex class II A gene polymorphism in the striped bass

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Abstract

Adaptions of the polymerase chain reaction were used to isolate cDNA sequences encoding the Major histocompatibility complex(Mhc) class II A gene(s) of the striped bass (Morone saxatilis). Four complete Mhc class II A genes were cloned and sequenced from a specimen originating in the Roanoke River, North Carolina, and another three A genes from a specimen originating from the Santee-Cooper Reservoir, South Carolina, identifying a total of seven unique sequences. The sequence suggests the presence of at least two Mhc class II A loci. The extensive sequence variability observed between the seven different Mhc class II clones was concentrated in the α1 encoding domain. The encoded α2, transmembrane, and cytoplasmic regions of all seven striped genes correlated well with those of known vertebrate Mhc class II proteins. Overall, the striped bass sequences showed greatest similarity to the Mhc class II A genes of the zebrafish. Southern blot analysis demonstrated extensive polymorphism in the Mhc class II A genes in members of a Roanoke river-caught population of striped bass versus a lesser degree of polymorphism in an aquacultured Santee-Cooper population of striped bass.

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Authors and Affiliations

  1. Department of Biology, Howell Science Complex, East Carolina University, 27858-4353, Greenville, NC, USA

    J. J. Hardee, U. Godwin, R. Benedetto & T. J. McConnell

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  1. J. J. Hardee
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  2. U. Godwin
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  3. R. Benedetto
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  4. T. J. McConnell
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Additional information

The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers (Mosa-A-S5) L35062, (Mosa-A-S8) L35066, (Mosa-A-R7) L35067, and (Mosa-A-S7) L35072 L35066, (Mossa-A-R7) L35067, and (Mosa-A-S7) L35072

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Hardee, J.J., Godwin, U., Benedetto, R. et al. Major histocompatibility complex class II A gene polymorphism in the striped bass. Immunogenetics 41, 229–238 (1995). https://doi.org/10.1007/BF00172064

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  • DOI: https://doi.org/10.1007/BF00172064

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