Summary
For continuous production of the antibiotic nikkomycin immobilized cells have been used in a fluidized bed bioreactor. Cells of Streptomyces tendae were immobilized on sintered glass particles. Different biomass concentrations on the particles correspond to different thicknesses of mycelial layers because growth occurs only on the outer surface of the particles. The antibiotic productivity decreased with increasing layer thickness. In fermentations with higher concentrations of both biomass on the particles and dissolved oxygen levels of about 70% the productivity was also limited because of limited oxygen diffusion in the layers.
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References
Bayer T, Zhou W, Holzhauer K, Schügerl K (1989) Investigations of cephalosporin C production in an airlift loop reactor. Appl Microbiol Biotechnol 30:26–33
Behal V (1986) Enzymes of primary metabolism in antibiotic producing actinomycetes. In: Szabo G, Biro S, Goodfellow M (ed) Symposia biologica Hungarica, vol. 32. Biological, biochemical and biomedical aspects of actinomycetes. Akademiai Kiado, Budapest, pp 185–190
Berk D, Behie LA, Jones A, Lesser BH, Gaucher GM (1984) The production of the antibiotic patulin in a three phase fluidized bed reactor. I. Effect of medium composition. Can J Chem Eng 62:112–119
Brillinger GU (1979) Metabolic products of microorganisms. 181. Chitin synthase from fungi, a test model for substances with insecticidal properties. Arch Microbiol 121:71–74
Dähn U, Hagenmaier H, Höhne H, König WA, Wolf G, Zähner H (1976) Stoffwechselprodukte von Mikroorganismen, 154. Mitteilung. Nikkomycin, ein neuer Hemmstoff der Chitinsynthese bei Pilzen. Arch Microbiol 107:143–160
Deo YM, Gaucher GM (1984) Semicontinuous and continuous production of penicillin-G by Penicillium chrysogenum cells immobilized in κ-carragenan. Biotechnol Bioeng 26:285–295
Fiedler H-P, Kurth R, Langhärig J, Delzer J, Zähner H (1982) Nikkomycins: microbial inhibitors of chitin synthase. J Chem Technol Biotechnol 32:271–280
Henzler H-J (1982) Verfahrenstechnische Auslegungsgrundlagen für Rührbehälter als Fermenter. Chem Ing Technol 54:461–476
Hersbach GJM, Beek CP van der, Dijck PWM van (1984) The penicillins: properties, biosynthesis and fermentation. In: Vandamme EJ (ed) Biotechnology of industrial antibiotics. Drugs and pharmaceutical sciences, vol 22. Dekker, New York, pp 45–140
Hilgendorf P, Diekmann H, Thoma M (1987) Constant dissolved oxygen concentrations in cephalosporin C fermentation: applicability of different controllers and effect on fermentation parameters. Appl Microbiol Biotechnol 27:247–251
Khang YH, Shankar H, Senatore F (1988) Comparison of free and immobilized Cephalosporium acremonium for ß-lactam antibiotic production. Biotechnol Lett 10:719–724
Kim JH, Oh DK, Park SK, Park YH (1986) Production of penicillin in a fluidized-bed bioreactor using a carrier-supported mycelial growth. Biotechnol Bioeng 28:1838–1844
Morikawa Y, Karube I, Suzuki S (1980) Continuous production of bacitracin by immobilized living whole cells of Bacillus sp. Biotechnol Bioeng 22:1015–1023
Rollins MJ, Jensen SE, Westlake DWS (1988) Effect of aeration on antibiotic production by Streptomyces clavuligerus. J Ind Microbiol 3:357–364
Schepers E (1988) Immobilisierung von Streptomyces tendae auf Sinterglas und Untersuchungen zur kontinuierlichen Nikkomycinbildung mit immobilisierten Zellen. Dissertation, Tübingen
Schügerl K (1984) Fermentationssysteme für verschiedene Einsatzgebiete. In: Bundesministerium für Forschung und Technologie, Bonn (ed) 3. BMFT-Statusseminar Bioverfahrenstechnik Jülich 1984, pp 51–89
Trück HU, Chmiel H, Hammes WP, Trösch W (1990) A study of N- and P-dependence of nikkomycin production in continuous culture with immobilized cells. Appl Microbiol Biotechnol 33:139–144
Vandamme EJ, Leymann D, DeVisscher P, DeBuyser D (1981) Effect of aeration and pH on gramicidin S production by Bacillus brevis. J Chem Technol Biotechnol 31:247–257
Veelken M, Pape H (1982) Production of tylosin and nikkomycin by immobilized Streptomyces cells. Eur J Appl Microbiol Biotechnol 15:206–210
Wang DIC, Cooney CL, Demain AL, Dunnill P, Humphrey AE, Lilly M (1979) Fermentation and enzyme technology. Wiley, New York, pp 157–193
Webb C, Black GM, Atkinson B (1986) Process engineering aspects of immobilized cell systems. Pergamon Press, New York
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Uwe Trück, H., Chmiel, H., Hammes, W.P. et al. Effects of oxygen supply on the production of nikkomycin with immobilized cells of Streptomyces tendae . Appl Microbiol Biotechnol 34, 1–4 (1990). https://doi.org/10.1007/BF00170913
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DOI: https://doi.org/10.1007/BF00170913