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On the mechanism of direct conversion of cellulose to ethanol by Fusarium oxysporum: effect of cellulase and β-glucosidase

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Summary

The effects of the three main enzymes involved in cellulose saccharification, namely cellobiohydrolase, carboxymethylcellulase and β-glucosidase, on the direct conversion of cellulose to ethanol by Fusarium oxysporum F3 were investigated. Ethanol production was not affected when the activity of the former two enzymes was varied within a wide range. By contrast, β-glucosidase markedly affected ethanol production showing an optimum level of 0.7–0.8 unit/ml growth medium. A significant decrease of cellulose bioconversion time to ethanol was obtained when β-glucosidase activity was adjusted to this optimal level at the beginning of the fermentation process.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Ave., 15773, Polytechnioupolis Zografou, Greece

    Paul Christakopoulos, Basil J. Macris & Dimitris Kekos

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  1. Paul Christakopoulos
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  2. Basil J. Macris
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  3. Dimitris Kekos
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Christakopoulos, P., Macris, B.J. & Kekos, D. On the mechanism of direct conversion of cellulose to ethanol by Fusarium oxysporum: effect of cellulase and β-glucosidase. Appl Microbiol Biotechnol 33, 18–20 (1990). https://doi.org/10.1007/BF00170562

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  • DOI: https://doi.org/10.1007/BF00170562

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