Summary
Resting cells of the coryneform strain NTB-1, previously incorrectly classified as Alcaligenes denitrificans NTB-1, quantitatively converted 4-chlorobenzoate (4-CBA) to 4-hydroxybenzoate (4-HBA) under strict anaerobic conditions in the presence of ferricyanide or nitrate. 4-HBA formation was enhanced by supplying anaerobic cells with glucose as an energy source. Using permeabilized cells it was shown that energy is not needed to drive the energy-dependent uptake of 4-CBA but also to convert 4-CBA into 4-HBA. In extracts it was subsequently demonstrated that a coenzymeA-thioester of 4-CBA is involved in the metabolism of 4-CBA.
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Groenewegen, P.E.J., van den Tweel, W.J.J. & de Bont, J.A.M. Anaerobic bioformation of 4-hydroxybenzoate from 4-chlorobenzoate by the coryneform bacterium NTB-1. Appl Microbiol Biotechnol 36, 541–547 (1992). https://doi.org/10.1007/BF00170200
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DOI: https://doi.org/10.1007/BF00170200