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Rock phosphate solubilization by Aspergillus niger grown on sugar-beet waste medium

  • Environmental Biotechnology
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Abstract

Solubilization of rock phosphate by Aspergillus niger was studied in solid-state fermentation on sugar-beet waste. This combination was selected after testing three agroindustrial waste materials, namely rice hulls, sugar-beet waste and alperujo. Sugar-beet waste was the best substrate for fungal growth with 69% mineralization, followed by rice hulls and alperujo. The fungus was successfully cultivated on sugar-beet waste supplemented with 3.0 g/l rock phosphate, acidifying the medium and thus decreasing the pH to 3–3.5. Solubilization of insoluble phosphate increased during the first half of the process, reaching a maximum of 292 μg phosphate/ml, although a part of it was probably consumed by the mycelium.

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References

  • AOAC (1980) Official methods of analysis, 14th edn. Association of Official Analytical Chemists, Washington, DC

    Google Scholar 

  • Asea PEA, Kucey RMN, Stewart JWB (1988) Onorganic phosphate solubilization by two Penicillium species in solution culture and soil. Soil Biol Biochem 20:459–464

    Google Scholar 

  • Cerezine PC, Nahas E, Banzatto DA (1988) Soluble phosphate accumulation by Aspergillus niger from fluorapatite. Appl Microbiol Biotechnol 29:501–505

    Google Scholar 

  • Cunningham JE, Kuiack C (1992) Production of citric and oxalic acids and solubilization of calcium phosphate by Penicillium bilaii. Appl Environ Microbiol 52:1451–1458

    Google Scholar 

  • Czajkowska D, Hornecka D, Ilnicka-Olejniczak O (1988) Biosynthesis of cellulolitic enzymes by Aspergillus niger A.N. 33 and its selectants. Acta Biotechnol 8:349–355

    Google Scholar 

  • Earl K, Syers J, McLaughlin RM (1979) Origin of the effect of citrate, tartarate, and acetate on phosphate sorption by soils and synthetic gels. Soil Sci Soc Am J 43:674–678

    Google Scholar 

  • Fox T, Comerford N (1990) Low-molecular weight acids in selected forest soils of the southeastern USA. Soil Sci Soc Am J 54:1139–1144

    CAS  Google Scholar 

  • Gerke L (1992) Phosphate, aluminium, and iron in the soil solution of three different soils in relation to varying concentrations of citric acid, z Pflanzenernahr Bodenk 155:339–343

    Google Scholar 

  • Goering HK, Van Soest PJ (1970) Forage fibre analysis. Agriculture Handbook 379. Agriculture Research Service, USDA, Washington, DC, pp 1–12

    Google Scholar 

  • Gomes J, Esterbauer H, Gomes I, Steiner W (1989) Screening of some wild fungal isolates for cellulolytic activities. Lett Appl Microbiol 8:67–70

    Google Scholar 

  • Hayman DS (1975) Phosphorus cycling by soil microorganisms and plant roots. In: Walker N (ed) Soil microbiology. Butterworth, London, pp 67–91

    Google Scholar 

  • Juven B, Henis Y (1970) Antimicrobial activity of olive phenolic compounds. J Appl Bacteriol 33:723–725

    Google Scholar 

  • Khan MAA, Hussain MM, Khalique MA, Rahman MA (1970) Methods of citric acid fermentation from molasses by Aspergillus niger. Pak J Sci Ind Res 13:439–445

    Google Scholar 

  • Khasawneh FE, Doll EC (1978) The use of phosphate rock for direct application to soils. Adv Agron 30:159–206

    Google Scholar 

  • Kucey RMN (1987) Increased phosphorus uptake by wheat and field beans inoculated with a phosphorus-solubilizing Penicillium bilaii and with mycorrhizal fungi. Appl Environ Microbiol 55: 2699–2703

    Google Scholar 

  • Kumar N, Sign K (1990) Chemical and microbiological changes during solid substrate fermentation of wheat straw with Coprinus fimetatus. Biol Wastes 33:231–242

    Google Scholar 

  • Lachica M, Aguilar A, Yanez J (1973) Analisis foliar. Metodos analiticos utilizados en la Estacion Experimental del Zaidin. An Edafol Agrobiol 32:1033–1047

    Google Scholar 

  • Lakshminarayana K, Chandhary K, Ethiraj S, Tauro P (1975) Solid state fermentation method for citric acid production using sugar cane bagasse. Biotechnol Bioeng 17:291–293

    Google Scholar 

  • Martin SM, Steel R (1955) Effect of phosphate on the production of organic acids. Can J Microbiol 1:470–474

    Google Scholar 

  • Mattey M (1992) The production of organic acids. Rev Biotechnol 12:87–132

    Google Scholar 

  • Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    CAS  Google Scholar 

  • Nahas E, Banzatto DA, Assis LC (1990) Fluorapatite solubilization by Aspergillus niger in vinasse medium. Soil Biol Biochem 22:1097–1101

    Google Scholar 

  • Oriol E, Schettino B, Vinegra-Gonzales G, Raimbault M (1988) Solid state culture of Aspergillus niger on support. J Ferment Technol 66:57–62

    Google Scholar 

  • Ozanne PG (1980) In: Kasawneh FE, Sample EC, Kamprath EL (eds) The role of phosphorus in agriculture. American Society of Agronomy, Madison USA, pp 559–589

    Google Scholar 

  • Taussky HH (1949) A microcolorimetric method for the determination of citric acid. J Biol Chem 181:195–198

    Google Scholar 

  • Vassilev N, Ganchev I (1986) Selection of Aspergillus niger strains for citric acid productivity. Acta Microbiol Bulg 18:68–72

    Google Scholar 

  • Vassilev N, Vassileva M (1992) Production of organic acids by immobilized filamentous fungi. Mycol Res 96:563–570

    Google Scholar 

  • Vassilev N, Vassileva M, Ganchev I (1986) Citric acid production by Aspergillus niger on starch hydrolysate media. Acta Microbiol Bulg 18:62–67

    Google Scholar 

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Vassilev, N., Baca, M.T., Vassileva, M. et al. Rock phosphate solubilization by Aspergillus niger grown on sugar-beet waste medium. Appl Microbiol Biotechnol 44, 546–549 (1995). https://doi.org/10.1007/BF00169958

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  • DOI: https://doi.org/10.1007/BF00169958

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