Summary
The effect of initial concentrations of malate varying from 0 to 28.6 g/l was studied. The acid was found to be inhibitory for growth of Schizosaccharomyces pombe but not for its deacidification activity. Malate was never integrated into biomass but partly transformed into ethanol if the aeration rate was weak (oxygen limitation). In the absence of glucose, resting cells of S. pombe were able to degrade malic acid if their concentration was sufficient, but their viability gradually decreased. However, for 0.15 g/l of growing cells (inoculum) 6 g/l of glucose was necessary to consume 8 g/l of malate. When the medium did not contain sugar no growth was observed despite the partial consumption of malate, showing that the acid was neither a carbon source nor an energy source.
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References
Benda I (1973) Les Schizosaccharomyces et leurs effects désacidifiants en vinification. In: ITV (eds) Colloque International d'Oenologie d'Arc et Senans, Doubs. Vigne et Vins ITV, Paris, p 31
Charpentier C, Feuillat M, Gerbaux V, Auther R (1985) La désacidification biologique des vins blancs par les Schizosaccharomyces. CR Agric France 71, 4:425–432
De Queiros H, Pareilleux A (1990) Growth kinetics of Schizosaccharomyces pombe under various culture conditions: influence of pH, malate ethanol and oxygenation. Appl Microbiol Biotechnol 33:578–581
Dittrich HH (1963) Zum chemismus des apfelsäureabbaues mit einer hefe der gattung Schizosaccharomyces. Wein-Wiss 18:406–411
Kunkee RE (1967) Malolactic fermentation. Adv Appl Microbiol 9:235–300
Maconi E, Manachini PL, Aragozzini F, Gennari C, Ricca GS (1984) A study of the maloalcoholic fermentation pathway in S. pombe. Biochem J 217:585–588
Magyar I, Panyik I (1989) Biological deacidification of wine by Schizosaccharomyces pombe entrapped in Ca-alginate gel. Am J Enol Vitic 40, 4:233–240
Mayer K, Temperli A (1963) The metabolism of 1-malate and others compounds by Schizosaccharomyces pombe. Archiv Mikrobiol 46:321–328
Osothsilp C, Subden RC (1986) Malate transport in S. pombe. J Bacteriol 168 3:1439–1443
Peynaud E, Domercq S, Boidron AM, Lafon-Lafourcade S, Guimberteau G (1964) Etude des levures Schizosaccharomyces pombe métabolisant l'acide 1-malique. Archiv Mikrobiol 48:150–165
Snow PG, Gallander JF (1979) Deacidificationof white table wines through partial fermentation with S. pombe. Am J Enol Vitic 30, 1:45–48
Taillandier P, Goma G, Strehaiano P (1988a) Les Schizosaccharomyces en vinification: levures indésirables ou facteurs de qualité? Microbiologie Aliments Nutrition 6:69–76
Taillandier P, Riba JP, Strehaiano P (1988b) Malate utilisation by Schizosaccharomyces pombe. Biotechnol Lett 10:469–472
Vezinhet F, Barre P (1982) Action de quelques facteurs du milieu sur le métabolisme de l'acide malique par Saccharomyces et Schizosaccharomyces au cours de la fermentation alcoolique. Sci Aliment 2:297–312
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Taillandier, P., Strehaiano, P. The role of malic acid in the metabolism of Schizosaccharomyces pombe: substrate consumption and cell growth. Appl Microbiol Biotechnol 35, 541–543 (1991). https://doi.org/10.1007/BF00169765
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DOI: https://doi.org/10.1007/BF00169765