Summary
An automated turbidimetric immunoassay for pullulanase from Clostridium thermosulfurogenes was developed for on-line bioprocess monitoring. Measurements are based on the turbidity caused by specific aggregation between pullulanase molecules present in the cultivation medium and antibodies against these enzymes. The time for one assay cycle is 3.0 min. Pullulanase concentrations between 10 units (U)/l and 1000 U/l could be measured. Standard deviations of less than 2% were found. The analysis system could be successfully employed for on-line product monitoring during a 240-h cultivation of C. thermosulfurogenes. An average correlation factor of 0.975 was obtained for the pullulanase data from on-line turbidimetric and the off-line reference assay, demonstrating the accuracy of this on-line method.
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Freitag, R., Scheper, T., Spreinat, A. et al. On-line monitoring of pullulanase production during continuous culture of Clostridium thermosulfurogenes . Appl Microbiol Biotechnol 35, 471–476 (1991). https://doi.org/10.1007/BF00169752
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DOI: https://doi.org/10.1007/BF00169752