Summary
In saphenous nerve neuromata of adult rats a long-term increase of immunoreactive nerve growth factor (irNGF) was detected after nerve transection. The occurrence of messenger RNA encoding NGF (NGF mRNA) in these proximal nerve stumps indicates local biosynthesis of NGF. In situ superfusion of neuromata revealed a constant release of irNGF which was significantly reduced by substance P (SP) but not affected by calcitonin gene related peptide (CGRP). We therefore suggest that SP may modulate the availability of NGF in the microenvironment of the regenerating nerve fiber endings.
References
Cheley S, Anderson R (1984) A reproducible microanalytical method for the detection of specific sequences by dot-blot hybridization. Anal Biochem 137:15–19
Davies AM, Bandtlow C, Heumann R, Korsching S, Rohrer H, Thoenen H (1987) Timing and site of nerve growth factor synthesis in developing skin in relation to innervation and expression of the receptor. Nature 326:353–358
Hartung HP, Wolters K, Toyka KV (1986) Substance P: binding properties and studies on cellular responses in guinea pig macrophages. J Immunol 136:3856–3863
Heumann R, Korsching S, Scott J, Thoenen H (1984) Relationship between levels of nerve growth factor (NGF) and its messenger RNA in sympathetic ganglia and peripheral target tissues. EMBO J 3 (no. 13):3183–3184
Heumann R, Korsching S, Bandtlow C, Thoenen H (1987) Changes of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transection. J Cell Biel 104:1623–1631
Hökfelt T, Millhorn D, Seroogy K, Tsuruo Y, Ceccatelli S, Lindh B, Meister B, Melander T, Schalling M, Bartfai T, Terenias L (1987) Coexistence of peptides with classical neurotransmitter. Experientia 43:768–780
Holzer-Petsche U, Schimek E, Amann R, Lembeck F (1985) In vivo and in vitro actions of mammalian tachykinins. NaunynSchmiedeberg's Arch Pharmacol 330:130–135
Jessell T, Tsunoo A, Kanazawa I, Otsuka M (1979) Substance P: depletion in the dorsal horn of rat spinal cord after section of the peripheral processes of the primary sensory neurons. Brain Res 168:247–259
Lee Y, Takami K, Kawai Y, Girgis S, Hillyard CJ, Mac Intyre I, Emson PC, Tohyama M (1985) Distribution of calcitonin generelated peptide in the rat peripheral nervous system with reference to its coexistence with substance P. Neuroscience 15:1227–1237
Lehrach H, Diamond D, Wozney JM, Boedtker H (1977) RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16:4743–4751
Lembeck F, Gamse R (1982) Substance P in peripheral sensory processes. In: Substance P in the nervous system. Pitman, London (Ciba Found Symp 91), pp 35–54
Levi-Montalcini R, Angeletti PU (1968) Nerve growth factor. Physiol Rev 18:534–569
Melton DA, Krieg PA, Regliabatti MR, Maniatis T, Zinn K, Green MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promotor. Nucleic Acids Res 12:7035–7056
Otten U (1984) Nerve growth factor and the peptidergic sensory neurons. Trends Pharmacol Sci 5:307–310
Otten U, Weskamp G (1987) Inflammatory stimuli trigger nerve growth factor synthesis in mononuclear cells. NaunynSchmiedeberg's Arch Pharmacol, Suppl 335: R73
Rush RA (1984) Immunohistochemical localization of endogenous nerve growth factor. Nature 312:364–367
Scott J, Selby M, Urdea M, Quiroga M, Bell GI, Rutter WJ (1983) Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factor. Nature 302:538–540
Senapati A, Anand P, McGregor GP, Ghatei MA, Thompson RPH, Bloom SR (1986) Depletion of neuropeptides during wound healing in rat skin. Neurosci Lett 71:101–105
Shebab SAS, Atkinson SE (1986) Vasoactive intestinal polypeptide (VIP) increases in the spinal cord after peripheral axotomy of the sciatic nerve originate from afferent neurons. Brain Res 372:37–44
Skofitsch G, Donnerer J, Petronijevic S, Saria A, Lembeck F (1983) Release of histamine by neuropeptides from the perfused rat hindquarter. Naunyn-Schmiedeberg's Arch Pharmacol 322: 153–157
Thoenen H, Barde YA (1980) Physiology of nerve growth factor. Physiol Rev 60:1284–1335
Weskamp G, Lorez HP, Keller HH, Otten U (1986) Cholinergic but not aminergic denervation increases nerve growth factor content in the adult rat hippocampus and cerebral cortex. Naunyn-Schmiedeberg's Arch Pharmacol 334:346–351
Weskamp G, Otten U (1987) An enzyme-linked immunoassay for nerve growth factor (NGF): a tool for studying regulatory mechanisms involved in NGF production in brain and peripheral tissues. J Neurochem 48:1779–1786
Author information
Authors and Affiliations
Additional information
Send offprint requests to U. Otten
Rights and permissions
About this article
Cite this article
White, D.M., Ehrhard, P., Hardung, M. et al. Substance P modulates the release of locally synthesized nerve growth factor from rat saphenous nerve neuroma. Naunyn-Schmiedeberg's Arch Pharmacol 336, 587–590 (1987). https://doi.org/10.1007/BF00169319
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00169319