Summary
Oncogenes play an important role in the process of malignant transformation. Since many of the protein tyrosine kinases (PTK) are products of oncogenes, the aim of this study was to demonstrate whether an increased PTK activity could be found in head and neck tumors. By using a non-radioactive dot-blot assay, PTK activity was measured in tumor and normal tissues of 38 patients with laryngeal cancer. The control group consisted of 19 healthy persons. PTK activity in tumor cells was significantly higher (P < 0.001) than in normal cells of the tumor patients and normal controls. Additionally, the PTK activity in the normal mucosa of the tumor patients was significantly higher than in the normal mucosa of the control group.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bergler W, Bier H, Ganzer U (1989) The expression of epidermal growth factor receptors in the oral mucosa of patients with oral cancer. Arch Otorhinolaryngol 246:121–125
Bishop JM (1987) The molecular genetics of cancer. Science 235:305–311
Druker BJ, Mamon HJ, Roberts TM (1989) Oncogenes, growthfactors and signal transduction. N Engl J Med 321:1383–1391
Eisbruch A (1987) Analysis of the epidermal growth factor receptor gene in fresh human head and neck tumors. Cancer Res 47:3603–3605
Erp HE van (1992) Protein tyrosine kinases in human brain, oligodendrogliomas and astrocytomas. J Neurochem 58:554–561
Field JK, Spandidos DA (1987) Expression of oncogenes in human tumours with special reference to the head and neck. J Oral Pathol 16:97–107
Field JK, Spandidos DA (1990) The role of ras and myc oncogenes in human solid tumours and their relevance in diagnosis and prognosis (review). Anticancer Res 10:1–22
Hennipman A, Oirschot BA van, Smits J, Rijksen G, Staal GEJ (1989) Tyrosine kinase activity in breast cancer, benign breast disease, and normal breast tissue. Cancer Res 49:516–521
Hermanek P, Sobin LH, UICC (International Union Against Cancer) (1987) TNM classification of malignant tumours. Springer, Berlin Heidelberg New York, pp 1–197
Hordijk GJ, Ravasz LA (1989) Head and neck cancer — a multidisciplinary problem (in Dutch). Scheltema and Holkema, Utrecht
Hunter T (1987) A thousand and one proteine kinases. Cell 50:823–829
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with the folin reagent. J Biol Chem 312: 146–151
Maxwell SA, Sacks PG, Gutterman JU, Gullick GE (1989) Epidermal growth factor receptor protein-tyrosine kinase activity in human cell lines established from squamous carcinomas of the head and neck. Cancer Res 49:1130–1137
Merritt WD, Weissler MC, Turk BF, Gilmer TM (1990) Oncogene amplification in squamous cell carcinoma of the head and neck. Arch Otorhinolaryngol 116:1394–1398
Ottenhoff-Kalff AE, Rijksen G, Beurden EACM van, Schipper-Kester C, Adriaansen-Slot SS, Hennipman A, Michels AA, Staal GEJ (1992) Characterization of protein tyrosine kinases from human breast cancer; involvement of the c-src oncogene product. Cancer Res 52:4773–4778
Partridge M, Gullick WJ, Langdon JD, Scherriff M (1982) Expression of epidermal growth factor receptor on oral squamous cell carcinoma. Br J Oral Max Surg 26:381–389
Rijksen G, Oirschot BA van, Staal GEJ (1991) Nonradioactive assays of phospho-tyrosine kinase activity using anti-phosphotyrosine antibodies. In: Hunter T, Sefton BM (eds) Methods in Enzymology, vol 200. Protein phosphorylation, part A. Academic Press, San Diego, pp 98–106
Riviére A, Wilckens C, Löning T (1990) Expression of c-erb B2 and c-myc in squamous epithelia and squamous cell carcinomas of the head and neck and the lower genital tract. J Oral Pathol Med 19:408–413
Ropka ME, Goodwin J, Levine PA, Sasaki CT, Kirchner JC, Cantrell RW (1991) Effective head and neck tumor markers. Arch Otolaryngol Head Neck Surg 117:1011–1014
Rydell EW, Olofsson J, Hellem H, Axelsson KL (1991) Tyrosine kinase activities in normal and neoplastic epithelia tissue of the human upper aero-digestive tract. Second Messengers Phosphoproteins 13:217–229
Scambia G, Panici PD, Battaglia F, Ferrandina G, Almadori G, Paludetti G, Maurizi M, Mancuso S (1991) Receptors for epidermal growth factor and steroid hormones in primary laryngeal tumors. Cancer 67:1347–1351
Slamon DJ, Kernion JB de, Verma IM, Cline MJ (1984) Expression of cellular oncogenes in human malignancies. Science 224:256–262
Terhaard CHJ (1991) Radiotherapy for the cancer of the larynx: treatment results and prognostic factors. Thesis, Utrecht
Ullrich A, Schlessinger J (1990) Signal transduction by receptors with tyrosine kinase activity. Cell 61:203–212
Author information
Authors and Affiliations
Additional information
Correspondence to: H. P. Verschuur
Rights and permissions
About this article
Cite this article
Verschuur, H.P., Rijksen, G., Schipper-Kester, G.P.M. et al. Protein tyrosine kinase activity in laryngeal squamous cell carcinoma. Eur Arch Otorhinolaryngol 249, 466–469 (1993). https://doi.org/10.1007/BF00168856
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00168856