Summary
Specific laccase activity was detected on SDS-PAGE using 2,2′-Azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid), guaiacol and syringaldazine as substrates. Enzyme activity was detected immediately following electrophoresis, after the detergent diffused from the gel into a renaturation buffer and subsequent Coomassie blue staining. Identification of laccase in a protein mixture and estimation of its molecular weight were done simultaneously.
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References
Lacks, S. A., Springhorn, S. S. (1980), The J. of Biological Chemistry 255, pp 7467–7473.
Laemmli, U. K. (1970), Nature (London) 227, pp 680–685.
Tanenbaum, S. W., Slomczynski, D., Nakas, J. P. (1995), Appl. Environm. Microbiol. 61, pp 907–912.
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Gonçalves, M.L.F.C., Steiner, W. Detection of laccase activity in polyacrylamide gels after electrophoresis under denaturing conditions. Biotechnol Tech 10, 667–668 (1996). https://doi.org/10.1007/BF00168476
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DOI: https://doi.org/10.1007/BF00168476