Summary
The modulation of the histamine release from histaminergic neurons by noradrenergic neurons was investigated by the push-pull technique.
The posterior hypothalamus of the conscious, freely moving rat was superfused with artificial CSF through a push-pull cannula and the release of endogenous histamine was determined in the superfusate. Hypothalamic superfusion with a potassium-rich CSF enhanced the release rate of histamine. Superfusion with the α2-agonists noradrenaline or clonidine diminished the release rate of histamine. Moreover, clonidine abolished the potassium-induced increase in the histamine release. Superfusion with the α2-antagonists yohimbine or idazoxan enhanced the release rate of histamine.
It is concluded that noradrenaline released from noradrenergic neurons of the hypothalamus modulates the release of histamine from histaminergic neurons by stimulating α2-adrenoreceptors located on histaminergic nerve terminals.
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References
Arrang JM, Garbarg M, Schwartz JC (1983) Auto-inhibition of brain histamine release mediated by a novel class (H3) of histamine receptor. Nature 302:832–837
Arrang JM, Garbarg M, Schwartz JC (1987) Auto-inhibition of histamine synthesis mediated by presynaptic H3-receptors. Neuroscience 23:149–157
Ericson H, Blomqvist A, Kohler C (1989) Brainstem afferents to the tuberomammillary nucleus in the rat brain with special reference to monoaminergic innervation. J Comp Neurol 281:169–192
Garbarg M, Tuong MDT, Gros CM, Schwartz JC (1989) Effects of histamine H3-receptor ligands on various biochemical indices of histaminergic neuron activity in rat brain. Eur J Pharmacol 164:1–11
Gulat-Marnay C, Lafitte A, Arrang JM, Schwartz JC (1989) Modulation of histamine release and synthesis in the brain mediated by α2-adrenoceptors. J Neurochem 53:519–524
Hill SJ, Straw RM (1988) α2-Adrenoceptor-mediated inhibition of histamine release from rat cerebral cortical slices. Br J Pharmacol 95:1213–1219
Klüver H, Barrera E (1953) A method for the combined straining of cells and fibers in the nervous system. J Neuropath Exp Neurol 12:400–403
Paxinos G, Watson C (1986) The rat brain in stereotaxic coordinates. Academic Press, New York
Philippu A (1984) Use of push-pull cannulae to determine the release of endogenous neurotransmitters in distinct brain areas of anaesthetized and freely moving animals. In: Marsden CA (ed) Measurement of neurotransmitter release in vivo. John Wiley, Chichester, pp 3–37
Philippu A, Died H, Sinha JN (1979) In vivo release of endogenous catecholamines in the hypothalamus. Naunyn-Schmiedeberg's Arch Pharmacol 308:137–142
Prast H, Saxer A, Philippu A (1988) Pattern of in vivo release of endogenous histamine in the mamillary body and the amygdala. Naunyn-Schmiedeberg's Arch Pharmacol 337:53–57
Tuomisto L, Yamatodani A, Died H, Waldmann U, Philippu A (1983) In vivo release of endogenous catecholamines, histamine and GABA in the hypertensive rats. Naunyn-Schmiedeberg's Arch Pharmacol 323:183–187
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This work was supported by the Fonds zur Förderung der wissenschaftlichen Forschung
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Prast, H., Heistracher, M. & Philippu, A. In vivo modulation of the histamine release in the hypothalamus by adrenoreceptor agonists and antagonists. Naunyn-Schmiedeberg's Arch Pharmacol 344, 183–186 (1991). https://doi.org/10.1007/BF00167216
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DOI: https://doi.org/10.1007/BF00167216