Abstract
The correlation between electrotransformation and electropermeability was studied in yeast cells following the modification of their membranes by treatment with n-alcohols. It was found that the number of transformed cells decreased with increase of chain length of the alcohols used as follows: methanol<ethanol<propanol<butanol. The electropermeability was unaffected by the prepulse n-alcohol treatment. The lack of a unidirectional link between permeability and transformation leads to the assumption that the mechanism of DNA introduction into the cell could not be interpreted solely as a result of the existence of pores.
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Ganeva, V.J., Tsoneva, I.C. Effect of n-alcohols on the electrotransformation and permeability of Saccharomyces cerevisiae . Appl Microbiol Biotechnol 38, 795–798 (1993). https://doi.org/10.1007/BF00167147
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DOI: https://doi.org/10.1007/BF00167147