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Malignant properties of P635 glioma are independent of glial fibrillary acidic protein expression

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Summary

To examine the relationship between the malignant behavior of rat glioma cells and expression of the differentiation antigen glial fibrillary acidic protein (GF), we assayed the tumorigenicity and metastatic ability of P635 and its GF + or GF − clones. We injected P635 (GF+) and clone 45 (GF−) cells intramuscularly in nude mice. Both lines formed local tumors which metastasized to lungs with equal efficiency. We then injected these lines intravenously in nude mice. Both produced experimental metastases in lungs and other organs with equal efficiency. Finally we injected P635 and several GF+ and GF− clones into the chorioallantoic membrane veins of naturally immune-deficient chick embryos and measured cell growth in embryonic liver. We again found that all clones survived and grew equally well. P635 cells are capable of extracranial growth and metastasis, two important features of the malignant phenotype. We conclude that GF in P635 is a neutral marker with respect to tumorigenicity and metastatic ability.

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Authors and Affiliations

  1. Department of Oncology, University of Western Ontario, London, Ontario, Canada

    Ann F. Chambers & J. Gregory Cairncross

  2. Department of Clinical Neurological Sciences, University of Western Ontario, London, Ontario, Canada

    J. Gregory Cairncross

  3. Department of Microbiology & Immunology, University of Western Ontario, London, Ontario, Canada

    Ann F. Chambers & J. Gregory Cairncross

  4. London Regional Cancer Center, 790 Commissioners Road, East, N6A 4L6, London, Ontario, Canada

    Ann F. Chambers, Georgetta H. Denhardt, Sylvia M. Wilson & J. Gregory Cairncross

Authors
  1. Ann F. Chambers
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  2. Georgetta H. Denhardt
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  3. Sylvia M. Wilson
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  4. J. Gregory Cairncross
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Chambers, A.F., Denhardt, G.H., Wilson, S.M. et al. Malignant properties of P635 glioma are independent of glial fibrillary acidic protein expression. J Neuro-Oncol 11, 43–48 (1991). https://doi.org/10.1007/BF00166996

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