Skip to main content
SpringerLink
Log in

Effect of amino acids on rapamycin biosynthesis by Streptomyces hygroscopicus

  • Original Paper
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

In a chemically defined medium containing aspartate, arginine and histidine to support good growth, addition of L-lysine stimulated rapamyycin production by 150%. This was probably due to its conversion to pipecolic acid, a rapamycin precursor. Phenylalanine and methionine interfered in rapamycin production by unknown mechanisms.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Brana AF, Demain AL (1988) Nitrogen control of antibiotic biosynthesis in actinomycetes. In: Sanchez-Esquivel S (ed) Nitrogen source control of microbiol processes. CRC Press, Boca Raton, Fla, pp 99–119

    Google Scholar 

  • Cheng YR, Hauck L, Demain AL (1995) Phosphate, ammonium, magnesium and iron nutrition of Streptomyces hygroscopicus with respect to rapamycin biosynthesis. J Ind Microbiol (in press)

  • Favret ME, Boeck LD (1992) Effect of cobalt and cyanocobalamin on biosynthesis of A10255, a thiopeptide antibiotic complex. J Antibiot (Tokyo) 45:1809–1811

    Google Scholar 

  • Gairola C, Hurley L (1976) The mechanism for the methionine mediated reduction of anthramycin yields in Streptomyces refuineus fermetations. Eur J Appl Microbiol 2:95–101

    Google Scholar 

  • Kojima I, Cheng YR, Mohan V, Demain AL (1995) Carbon source nutrition of rapamycin biosynthesis in Streptomyces hygroscopicus. J Ind Microbiol (in press)

  • Lam KS, Veitch JA, Golik J, Krishnan B, Klohr SE, Volk KJ, Forenza S, Doyle TW (1993) Biosynthesis of esperamicin A 1, an enediyne antitumor antibiotic. J Am Chem Soc 115: 12340–12345

    Google Scholar 

  • Omura S, Tanaka Y (1984) Biochemistry, regulation and genetics of macrolide production. In: Omura S (ed) Macrolide antibiotics: chemistry, biology, and practice. Academic Press, New York, pp 199–229

    Google Scholar 

  • Paiva NL, Demain AL, Roberts MF (1991). Incorporation of acetate, propionate, and methionine into rapamycin by Streptomyces hygroscopicus. J Nat Prod 54:167–177

    Google Scholar 

  • Paiva NL, Demain AL, Roberts MF (1993) The immediate precursor of the nitrogen-containing ring of rapamycin is free pipecolic acid. Enzyme Microb Technol 15:581–585

    Google Scholar 

  • Uyeda M, Demain AL (1988) Methionine inhibition of thienamycin formation. J Ind Microbiol 3:57–59

    Google Scholar 

Download references

Author information

Author notes

  1. Y. R. Cheng

    Present address: Fujian Institute of Microbiology, Fuzhou, People's Republic of China

Authors and Affiliations

  1. Fermentation Microbiology Laboratory, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA

    Y. R. Cheng, A. Fang & A. L. Demain

Authors
  1. Y. R. Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. L. Demain
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cheng, Y.R., Fang, A. & Demain, A.L. Effect of amino acids on rapamycin biosynthesis by Streptomyces hygroscopicus . Appl Microbiol Biotechnol 43, 1096–1098 (1995). https://doi.org/10.1007/BF00166931

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00166931

Keywords

Search

Navigation