Abstract
The psychrotrophic bacterium Pseudomonas fluorescens is a milk contaminant known to secrete a lipase that is a nuisance for the dairy industry but may have a biotechnological interest. Strain MFO secretes this enzyme upon induction under various conditions. Regardless of the inducer and growth temperature, a single enzyme is produced. However, optimal production occurs when the culture is grown at 17.5° C. Other exported proteins (an extracellular protease and two periplasmic phosphatases) have previously been shown to display exactly the same optimal temperature of production. In contrast, constitutive cell-bound esterase and cytochrome oxidase are produced at a roughly constant rate regardless of the growth temperature. The relevance of these results are discussed in terms of multifunctional regulation and interest for the dairy industry.
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Correspondence to: N. Orange
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Merieau, A., Gugi, B., Guespin-Michel, J.F. et al. Temperature regulation of lipase secretion by Pseudomonas fluorescens strain MFO. Appl Microbiol Biotechnol 39, 104–109 (1993). https://doi.org/10.1007/BF00166857
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DOI: https://doi.org/10.1007/BF00166857