Summary
The effect of pH and temperature on the growth and acidification characteristics of Streptococcus thermophilus 404 and Lactobacillus bulgaricus 398 were studied in order to optimize starter production. A quadratic two-variable model for each of these parameters is proposed. Optimal growth conditions were found at pH 6.5 and 40° C for S. thermophilus and pH 5.8 and 44° C for L. bulgaricus. Maximum acidification was obtained at pH values and temperatures higher than the optimal growth conditions. In addition, the two strains were generally more sensitive to pH effect.
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References
Accolas JP, Bloquel R, Didienne R, Regnier J (1977) Propriétés acidifiantes des bactéries lactiques thermophiles en relation avec la fabrication du voghourt. Lait 57:1–23
Accolas JP, Hemme D, Desmazeaud MJ, Vassal L, Bouillanne C, Veaux M (1980) Les levains lactiques thermophiles: propriétés et comportement en technologie laitiére. Lait 60:487–524
Andreyeva LN, Biryukov VV (1973) Analysis of mathematical models of the effect of pH on fermentation processes and their use for calculating optimal fermentation conditions. Biotechnol Bioeng Symp 4:61–76
Cochran WG, Cox GM (1957) Experimental designs, 2nd edn. Wiley, New York, pp 346–349
Eroshin VK, Utkin IS, Ladynichev SV, Samoylov VV, Kuvshinnikov VD, Skriabin GK (1976) Influence of pH and temperature on the substrate yield coefficient of yeast growth in a chemostat. Biotechnol Bioeng 18:289–295
Famelart MH, Kobilinsky A, Bouillane C, Desmazeaud MJ (1987) Influence of temperature, pH and dissolved oxygen on growth of Brevibacterium linens in a fermentor. Appl Microbiol Biotechnol 25:442–448
Lallai A, Mura G, Miliddi R, Mastinu C (1988) Effect of pH on growth of mixed cultures in batch reactor. Biotechnol Bioeng 31:130–134
Lebreton JD, Millier C (1982) Modéles dynamiques déterministes en biologie. Masson, Paris, pp 163–164
Martley FG (1983) Temperature sensitivities of thermophilic starter strains. NZJ Dairy Sci Technol 18:191–196
O'Leary VS, Woychik JH (1976) Utilisation of lactose, glucose and galactose by a mixed culture of Streptococcus thermophilus and Lactobacillus bulgaricus in milk treated with lactase enzyme. Appl Environ Microbiol 32:89–94
Oner MD, Erickson LE (1986) Anaerobic fermentation of Lactobacillus bulgaricus and Streptococcus thermophilus on 3% non-fat dry milk in pure and mixed cultures. Biotechnol Bioeng 28:883–894
Radke-Mitchell LC, Sandine WE (1986) Influence of temperature on associated growth of Streptococcus thermophilus and Lactobacillus bulgaricus. J Dairy Sci 69:2558–2568
Robinson RK (1988) Cultures for yogurt. Their selection and use. Dairy Ind Int 53:15–19
Stadhouders J, Leenders GJM (1984) Spontaneously developed mixed-strain cheese starters. Their behaviour towards phages and their use in the Dutch cheese industry. Neth Milk Dairy J 38:157–181
Tayeb J, Bouillanne C, Desmazeaud MJ (1984) Computerized control of growth with temperature in a mixed culture of lactic acid bacteria. J Ferment Technol 62:461–470
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Beal, C., Louvet, P. & Corrieu, G. Influence of controlled pH and temperature on the growth and acidification of pure cultures of Streptococcus thermophilus 404 and Lactobacillus bulgaricus 398. Appl Microbiol Biotechnol 32, 148–154 (1989). https://doi.org/10.1007/BF00165879
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DOI: https://doi.org/10.1007/BF00165879