Summary
An Escherichia coli-Zymomonas mobilis shuttle vector was constructed from a 15.5 kb native plasmid of ZM6 00 and the E. coli plasmid, pBR329. Integrative transfer of this shuttle vector from E. coli to Z. mobilis was achieved with the aid of the mobilizing plasmid, pRK2013. The shuttle vector was stable in Z. mobilis for at least 300 generations without antibiotic selection.
Similar content being viewed by others
References
Afendra AS, Drainas C (1987) Expression and stability of a recombinant plasmid in Zymomonas mobilis and Escherichia coli. J Gen Microbiol 133:127–134
Betlach M, Hershfield V, Chow L, Brown W, Goodman HM, Boyer HW (1976) A restriction endonuclease analysis of the bacterial plasmid controlling the EcoR1 restriction and modification of DNA. Fed Proc 35:2037–2043
Bolivar F, Backman K (1979) Plasmids of Echerichia coli as cloning vectors. In: Wu R (ed) Methods in enzymology, vol 68. Academic Press, New York, pp 245–267
Carey VC, Walia SK, Ingram LO (1983) Expression of a lactose transposon (Tn951) in Zymomonas mobilis. Appl Environ Microbiol 46:1163–1168
Conway T, Byun MOK, Ingram LO (1987) Expression vector for Zymomonas mobilis. Appl Environ Microbiol 53:235–241
Covarrubias L, Bolivar F (1982) Construction and characterization of new cloning vehicles VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplication. Gene 17:79–89
Covarrubias L, Cervantes L, Covarrubias A, Soberon X, Vichido I, Blanco A, Kupersztoch-Portnoy YM, Bolivar F (1981) Construction and characterization of new cloning vehicles V. Mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328. Gene 13:25–35
Crosa JH, Falkow S (1981) Plasmids. In: Gerhardt P (ed) Manual of methods for general bacteriology. American Society of Microbiology, Washington DC, pp 266–282
Eveleigh DE, Stokes HW, Dally EL (1983) Recombinant DNA approaches for enhancing the ethanol productivity of Zymomonas mobilis. In: Wise DL (ed) Organochemicals from biomass. Benjamin/Cummings, Menlo Park, California, pp 69–91
Figurski DH, Helinski DR (1979) Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci USA 76:1648–1652
Goodman AE (1985) Genetic manipulation of the bacterium Zymomonas mobilis. Ph. D. Thesis, University of New South Wales
Goodman AE, Strzelecki AT, Rogers PL (1984) Formation of ethanol from lactose by Zymomonas mobilis. J Biotechnol 1:219–228
Haffie TL, Louie PW, Khachatourians GG (1985) Isolation of noninhibitory strains of Zymomonas mobilis. Appl Environ Microbiol 49:1007–1009
Maniatis T, Fristch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Marko MA, Chipperfield R, Birnboim HC (1982) A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powder. Anal Biochem 121:382–387
Misawa N, Okamoto T, Nakamura K, Kitamura K, Yanase H, Tonomura K (1986) Construction of a new shuttle vector for Zymomonas mobilis. Agric Biol Chem 50:3201–3203
Murray NE, Brammar WJ, Murray K (1977) Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet 150:53–61
Rogers PL, Lee KJ, Skotnicki ML, Tribe DE (1982) Ethanol production by Zymomonas mobilis. Adv Biochem Eng 23:37–84
Skotnicki ML, Tribe DE, Rogers PL (1980) R plasmid transfer in Zymomonas mobilis. Appl Environ Microbiol 40:7–12
Skotnicki ML, Lee KJ, Tribe DE, Rogers PL (1982) Genetic alteration of Zymomonas mobilis for ethanol production. In: Hollaender A (ed) Genetic engineering of microorganisms for chemicals. Plenum Press, New York, pp 271–290
Skotnicki ML, Browne GM, Rogers PL (1984) Antibacterial activity of different Zymomonas mobilis strains. Microbios 39:187–192
Strzelecki AT, Goodman AE, Rogers PL (1986) Stability of a lac operon in Zymomonas mobilis in batch and continuous culture. J Biotechnol 3:197–205
Strzelecki AT, Goodman AE, Rogers PL (1987) Behaviour of the IncW plasmid Sa in Zymomonas mobilis. Plasmid 18:46–53
Swings J, De Ley J (1977) The biology of Zymomonas. Bacteriol Rev 41:1–46
Tomomura K, Okamoto T, Yasui M, Yanase H (1986) Shuttle vectors for Zymomonas mobilis. Agric Biol Chem 50:805–808
Author information
Authors and Affiliations
Additional information
Offprint requests to: S. F. Delaney
Rights and permissions
About this article
Cite this article
Cho, DW., Rogers, P.L. & Delaney, S.F. Construction of a shuttle vector for Zymomonas mobilis . Appl Microbiol Biotechnol 32, 50–53 (1989). https://doi.org/10.1007/BF00164822
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00164822