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The efficiency of the protein-dependent flocculation of Flavobacterium sp. is sensitive to the composition of growth medium

  • Applied Microbial and Cell Physiology
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Summary

The pure culture flocculation of Flavobacterium sp. strain 304 was shown to be limited to early stationary phase cells. The effects of proteolytic, cellulolytic and nucleolytic enzymes as well a agents such as divalent ions, citrate, malate, EDTA, poly-l-lysine, and NaN3 on flocculation was studied. The results indicate that the flocculation of strain 304 is protein mediated, and affected by composition of the growth medium. The primary interaction between the cells is not ionic or divalent cation dependent, but probably hydrophobic in nature. In addition, the role of single-species flocculation in activated sludge is discussed.

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References

  • Angelbeck DI, Kirch EJ (1969) Influence of pH and metal cations on aggregative growth of non-slime-forming strains of Zoogloea ramigera. Appl Microbiol 17:435–440

    Google Scholar 

  • Benedict RG, Carlson DA (1971) Aerobic heterotrophic bacteria in activated sludge. Water Res 5:1023–1030

    Google Scholar 

  • Deinema MH, Zevenhuizen PTM (1981) Formation of cellulose fibrils by Gram-negative bacteria and their role in bacterial flocculation. Arch Microbiol 78:42–57

    Google Scholar 

  • Easson DD Jr, Sinskey AJ, Peoples OP (1987) Isolation of Zoogloea ramigera I-16-M exopolysaccharide biosynthetic genes and evidence for instability within this region. J Bacteriol 169:4518–4524

    Google Scholar 

  • Endo T, Takahashi H (1980) Reconstitution of pronase-susceptible flocs of Flavobacterium sp. Agric Biol Chem 44:2413–2418

    Google Scholar 

  • Endo T, Takahashi H (1981a) Genetic evidence for the involvement of aggregation factor and other components in flocculation of Flavobacterium strain B. Agric Biol Chem 45:379–383

    Google Scholar 

  • Endo T, Takahashi H (1981b) Partial purification and some properties of aggregation factor from pronase-susceptible floc forming Flavobacterium strain B. Agric Biol Chem 45:513–516

    Google Scholar 

  • Endo T, Nakamura K, Takahashi H (1976) Pronase-susceptible floc-forming bacteria: relationship between flocculation and calcium ion. Agric Biol Chem 40:2289–2295

    Google Scholar 

  • Forster CF (1981) Activated sludge surfaces in relation to the sludge volume index. Water Res 5:861–870

    Google Scholar 

  • Forster CF (1985) Factors involved in the settlement of activated sludge — I. Nutrients and surface polymers. Water Res 19:1259–1264

    Google Scholar 

  • Friedman BA, Dugan PR, Pfister RM, Remsen CC (1968) Fine structure and composition of the zoogloeal matrix surrounding Zoogloea ramigera. J Bacteriol 96:2144–2153

    Google Scholar 

  • Friedman BA, Dugan PR, Pfister RM, Remsen CC (1969) Structure of exocellular polymers and their relationship to bacterial flocculation. J Bacteriol 98:1328–1334

    Google Scholar 

  • Gray NF (1989) Biology of wastewater treatment. Oxford University Press, Oxford, UK

    Google Scholar 

  • Hantula J, Kurki A, Vuoriranta P, Bamford DH (1991) Rapid classification of bacterial strains by SDS-polyacrylamide gel electrophoresis: population dynamics of the dominant dispersed phase bacteria of activated sludge. Appl Microbiol Biotechnol 34:551–555

    Google Scholar 

  • Hejzlar J, Chudoba J (1986) Microbial polymers in the aquatic environment — I. Production by activated sludge microorganisms under different conditions. Water Res 20:1209–1216

    Google Scholar 

  • Kakii K, Kitamura S, Shirakashi T, Kuriyama M (1985) Effect of calcium ion on sludge characteristics. J Ferment Technol 63:263–270

    Google Scholar 

  • Kakii K, Kitamura S, Shirakashi T, Kuriyama M (1986a) Comparison of mucilage polysaccharides extracted from sewage activated sludge. J Ferment Technol 64:51–56

    Google Scholar 

  • Kakii K, Sugahara E, Shirakashi T, Kuriyama M (1986b) Isolation and characterization of a Ca++-dependent floc-forming bacterium. J Ferment Technol 64:57–62

    Google Scholar 

  • Kato A, Izaki K, Takahashi H (1971) Floc-forming bacteria isolated from activated sludge. J Gen Appl Microbiol 17:439–456

    Google Scholar 

  • Krieg NR (1984) Bergey's manual of systematic bacteriology, vol 1. Williams and Wilkins, Baltimore

    Google Scholar 

  • Krul JM (1977) Some factors affecting floc formation by Zoogloea ramigera, strain I-16-M. Water Res 11:31–36

    Google Scholar 

  • Kurki A, Hantula J, Vuoriranta P, Bamford DH (1989) Floc formation mechanism of activated sludge bacteria. Water Sci Technol 21:1637–1638

    Google Scholar 

  • Maniatis T, Fritch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.

    Google Scholar 

  • Morgan JW, Forster CF, Evison L (1990) A comparative study of the nature of biopolymers extracted from anaerobic and activated sludges. Water Res 24:743–750

    Google Scholar 

  • Nishikawa S, Kuriyama M (1968) Nucleic acic as a component of mucilage in activated sludge. Water Res 2:811

    Google Scholar 

  • Olkkonen VM, Ojala PM, Bamford DH (1991) Generation of infectious nucleocapsids by in vitro assembly of the shell protein on to the polymerase complex of the dsRNA bacteriophage φ6. J Mol Biol 218:569–581

    Google Scholar 

  • Pavoni JL, Tenney MW, Echelberger WF Jr (1972) Bacterial exocellular polymers and biological flocculation. J Water Pollut Control Fed 44:414–431

    Google Scholar 

  • Sato T, Ose Y (1980) Floc-forming substances extracted from activated sludge by sodium hydroxide solution. Water Res 14:333–338

    Google Scholar 

  • Steiner AE, McLaren DA, Forster CF (1976) The nature of activated sludge flocs. Water Res 10:25–30

    Google Scholar 

  • Tago Y, Aida K (1975) The deflocculating enzyme produced by a floc-forming bacterium. J Gen Appl Microbiol 21:365–374

    Google Scholar 

  • Tago Y, Aida K (1977) Exocellular mucopolysaccharide closely related to bacterial floc formation. Appl Environ Microbiol 34:308–314

    Google Scholar 

  • Takiguchi Y (1972) Purification of the mucilage in activated sludge and its physico-chemical properties. J Ferment Technol 50:331–340

    Google Scholar 

  • Tenney MW, Verhoff FH (1973) Chemical and autoflocculation of microorganisms in biological wastewater treatment. Biotechnol Bioeng 15:1045–1073

    Google Scholar 

  • Tezuka Y (1967) Magnesium ion as a factor governing bacterial flocculation. Appl Microbiol 15:1256

    Google Scholar 

  • Tezuka Y (1969) Cation-dependent flocculation in a Flavobacterium species predominant in activated sludge. Appl Microbiol 17:222–226

    Google Scholar 

  • Unz RF, Farrah SR (1976) Exopolymer production and flocculation by Zoogloea MP6. Appl Environ Microbiol 31:623–626

    Google Scholar 

  • Witthauer DP (1980) Biocoenosis degradation in model wastewater treatment plants. Eur J Appl Microbiol Biotechnol 9:151–163

    Google Scholar 

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Hantula, J., Bamford, D.H. The efficiency of the protein-dependent flocculation of Flavobacterium sp. is sensitive to the composition of growth medium. Appl Microbiol Biotechnol 36, 100–104 (1991). https://doi.org/10.1007/BF00164707

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  • DOI: https://doi.org/10.1007/BF00164707

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