Abstract
The copolyester of 3-hydroxybutyrate and 3- hydroxyvalerate was synthesized from the combined carbon sources of glucose and sodium propionate by a filamentaion-defective mutant of Sphaerotilus natans, which is a typical filamentous bacterium often found in activated sludge. The 3-hydroxyvalerate content in the produced polymer increased with increasing concentrations of propionate. Cell growth and polyester synthesis were observed even when 0.6% sodium propionate was added to the medium, when the 3-hydroxyvalerate content in the polymer produced was about 60 mol%. The monomer composition of the copolymer was also varied by aeration conditions, time of propionate feeding, and cultivation time. This strain flocculated in accordance with cell growth, allowing rapid and convenient separation of the biomass from the culture fluid.
Similar content being viewed by others
References
Bloembergen S, Holden DA, Hamer GK, Bluhm TL, Marchessault RH (1986) Studies of composition and crystallinity of bacterial poly(β-hydroxybutyrate-co-β-hydroxyvalerate). Macromolecules 19:2865–2870
Bluhm TL, Hamer GK, Marchessault RH, Fyte CA, Veregin RP (1986) Isodimorphism in bacterial poly(β-hydroxybutyrate-co-hydroxyvalerate). Macromolecules 19:2871–2876
Byrom D (1987) Polymer synthesis by microorganisms:technology and economics. Trends Biotechnol 5:246–250
Comeau Y, Hall KJ, Oldham WK (1988) Determination of poly-β-hydroxybutyrate and poly-β-hydroxyvalerate in activated sludge by gas-liquid chromatography. Appl Environ Microbiol 54:2325–2327
Doi Y, Kunioka M, Nakamura Y, Soga K (1986) Nuclear-magnetic-resonance studies on poly(β-hydroxybutyrate) and a copolyester of β-hydroxybutyrate and β-hydroxyvalerate isolated from Alcaligenes eutrophus H16. Macromolecules 19:2860–2864
Doi Y, Tamaki A, Kunioka M, Soga K (1987) Biosynthesis of terpolyesters of 3-hydroxybutyrate, 3-hydroxyvalerate, and 5-hydroxyvalerate in Alcaligenes eutrophus from 5-chloropentanoic and pentanoic acids. Macromol Chem Rapid Commun 8:631–635
Eikelboom DH (1975) Filamentous organisms observed in activated sludge. Water Res 9:365–388
Holmes PA (1985) Applications of PHB — a microbially produced biodegradable thermoplastic. Physics Technol 16:32–36
Kim GJ, Yun KY, Bae KS, Rhee YH (1992) Accumulation of copolyestersconsisting of 3-hydroxybutyrate and 3-hydroxy-valerate by Alcaligenes sp. SH-69 in batch culture. Biotechnol Lett 14:27–32
Kunioka M, Tamaki A, Doi Y (1989) Crystalline and thermalproperties of bacterial copolymers — poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(β-hydroxybutyrate-co-4-hydroxybutyrate). Macromolecules 22:694–697
Mitomo H, Barham PJ, Keller A (1987) Crystallization and morphology of poly(β-hydroxybutyrate) and its copolymer. Polymer J 19:1241–1253
Oldham G, Tunlid A, Westerdahl G, Marden P (1986) Combined determination of poly-β-hydroxyalkanoic and cellular fatty acids in served marine bacteria and sewage sludge by gas chromatography with flame ionization or mass spectrometry detection. Appl Environ Microbiol 52:905–910
Poindexter JS, Eley LF (1983) Combined procedure for assays of poly-β-hydroxybutyric acid and inorganic polyphosphate. J Microbiol Methods 1:1–7
Ramzay BA, Ramzay JA, Cooper DG (1989) Production of poly-β-hydroxyalkanoic acid by Pseudomonas cepacia. Appl Environ Microbiol 55:584–589
Ramzay BA, Lomaliza K, Charvarie C, Dube B, Bataille P, Ramzay JA (1990) Production of poly-(β-hydroxybutyric-co-β-hydroxyvvaleric) acids. Appl Environ Microbiol 56:2093–2098
Satoh H, Mino T, Matsuo T (1992) Uptake of organic substrates and accumulation of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic conditions in the biological excess phosphate removal processesWater Sci Technol 26:933–942
Scandola M, Ceccorulli G, Doi Y (1990) Viscoelastic relaxations and thermal properties of bacterial poly(3-hydroxybutyrate-co-3-hydroxyvalera) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate). Int J Biol Macromol 12:112–117
Takeda M, Matsuoka H, Hamana H, Hikuma M (1995) Biosynthesis of poly-3-hydroxybutyrate by Spahaerotilus natans. Appl Microbiol Biotechnol 43:31–34
Tombolini R, Nuti MP (1989) poly(β-hydroxyalkanoate) biosynthesis and accumulation by different Rhizobium species. FEMS Microbiol Lett 60:299–304
Ueda S, Matsumoto S, Takagi A, Yamane T (1992a) n-Amyl alcohol as a substrate for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by bacteria. FEMS Microbiol Lett 98:57–60
Ueda S, Matsumoto S, Takagi A, Yamane T (1992b) Synthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from methanol and n-amyl alcohol by the methylotrophic bacteria Paracoccus denitrificans and Metylobacterium extorquens. Appl Environ Microbiol 58:3574–3579
Veen WL van, Mulder EG, Deinema MH (1978) The Sphaertilus-Leptothrix groups of bacteria. Microbiol Rev 42:329–356
Wallen LL, Rohwedder WK (1974) ploy-β-hydroxyalkanoate from activated sludge. Environ Sci Technol 8:576–578
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Takeda, M., Matsuoka, H., Ban, H. et al. Biosynthesis of poly(3-hydroxybutyrate-Co-3-hydroxyvalerate) by a mutant of Sphaerotilus natans . Appl Microbiol Biotechnol 44, 37–42 (1995). https://doi.org/10.1007/BF00164477
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00164477